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cDNA (Complementary DNA)

cDNA (Complementary DNA)

cDNA (Complementary DNA) Animation

Complementary DNA (cDNA) is a doublestranded DNA version of an mRNA molecule. In higher eukaryotes, an mRNA is a more useful predictor of a polypeptide sequence than is a genomic sequence, because the introns have been spliced out. Researchers prefer to use cDNA rather than mRNA itself because RNAs are inherently less stable than DNA and techniques for routinely amplifying and purifying individual RNA molecules do not exist.

The cDNA is made from mRNA with the use of a special enzyme called reverse transcriptase, originally isolated from retroviruses. Using an mRNA molecule as a template, reverse transcriptase synthesizes a single-stranded DNA molecule that can then be used as a template for double-stranded DNA synthesis. cDNA does not need to be cut in order to be cloned.

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  • Proteinase K solution 25ml (20mg/ml)

    Proteinase K solution 25ml (20mg/ml)

    €733.20
    Proteinase K is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic or hydrophobic amino acids. Proteinase K is classified as a serine protease. The smallest peptide to be hydrolysed is a tetrapeptide. DNA...
    503-PK
    €733.20
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  • Proteinase K Powder 200mg

    Proteinase K Powder 200mg

    €305.50
    Proteinase K is an endoproteolytic enzyme that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic or hydrophobic amino acids. Proteinase K is classified as a nonspecific serine protease. The smallest peptide to be hydrolysed is a...
    506-PKP
    €305.50
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  • Anti-PON1 Clone KRJ2

    Anti-PON1 Clone KRJ2

    €666.90 - €2,646.80
    PON1 is a polymorphic enzyme closely associated with high-density lipoproteins. It is located on chromosome 7 along with 2 other paraoxanase genes, PON2 and PON3. It is a 355 amino acid, 43 kDa glycoprotein produced in the liver and secreted into the...
    0801014/0801097
    €666.90 - €2,646.80
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    €666.90 - €2,646.80
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  • Anti-PON1 Clone KRJ1

    Anti-PON1 Clone KRJ1

    €666.90 - €2,646.80
    PON1 is a polymorphic enzyme closely associated with high-density lipoproteins. It is located on chromosome 7 along with 2 other paraoxanase genes, PON2 and PON3. It is a 355 amino acid, 43 kDa glycoprotein produced in the liver and secreted into the...
    0801012/0801095
    €666.90 - €2,646.80
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  • Anti-HTLV Type II p24 Clone 754.21.11

    Anti-HTLV Type II p24 Clone 75/4.21.11

    €666.90 - €2,646.80
    Anti-HTLV-II p24 (Clone 75/4.21.11) reacts with Human T-Lymphotropic Virus Type II (HTLV-II) core protein. This murine monoclonal antibody exhibits reactivity with viral lysates examined using Western blotting and ELISA techniques. The antibody...
    0801087/0801120
    €666.90 - €2,646.80
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    €666.90 - €2,646.80