HIV DNA RT-PCR Qual | R-V1-D

HIV DNA RT-PCR Qual | R-V1-D from Sacace Biotechnologies is available for delivery

Description:

General information: Real Time PCR Test for quantitative detection of HIV. Real Time PCR Test for quantitative detection of HIV

Target Disease Type: HIV Associated Kits

Specific Application: HIV DNA

Storage and Shipping : n request

HIV DNA RT-PCR Qual R-V1-D DataSheet

INTENDED USE

kit HIV DNA Real-TM is a Real-Time test for the Qualitative detection of the proviral DNA of human immunodeficiency virus (HIV) in whole blood.

PRINCIPLE OF ASSAY

Kit HIV DNA Real-TM is a Real-Time test for the Qualitative detection of Human Immunodeficiency Virus in whole blood. HIV DNA is extracted from blood, amplified using Real Time Amplification and detected using fluorescent reporter dye probes specific for HIV or HIV IC (β-globine gene).

HIV DNA Real-TM kit is a qualitative test based on the use of an endogenous control, the β-globin gene. The DNA target selected as an endogenous internal control is a human genome fragment that is present in sample in a sufficient quantity equivalent to that of cells in the sample. Internal Control (IC) serves as an amplification control for each individually processed specimen and to identify possible inhibition. IC is detected in a channel other than the HIV DNA.

Monitoring the fluorescence intensities during Real Time allows the detection of the accumulating product without having to re-open the reaction tube after the amplification. The kit will allow the detection of 100 samples.

STABILITY

HIV DNA Real-TM Test is stable up to the expiration date indicated on the kit label. The product will maintain performance through the control date printed on the label. Exposure to light, heat or humidity may affect the shelf life of some of the kit components and should be avoided.

Repeated thawing and freezing of these reagents should be avoided, as this may reduce the sensitivity. Components stored under conditions other than those stated on the labels may not perform properly and may adversely affect the assay results.