HDM Elisa & Assay Kits
House Dust Mite Related Reagents |
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Products |
Quantity |
Catalog # |
0.6 mg, lyophilized |
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0.6 mg, lyophilized |
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House Dust Mite Extract from D. pteronyssinus - Biotinylated |
0.1 mg, lyophilized |
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0.1 mg, lyophilized |
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0.5 mg/ml x 5 ml |
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Mouse Anti-HDM Antibody ELISA Kits |
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Mouse Anti-HDM Total Immunoglobulin ELISA Kits |
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House Dust Mite Allergen Detection ELISA Kit |
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Trypsin Activity Assay Kit |
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Allergies against house dust mites (HDM), a common aeroallergen, are observed in up to 85% of asthmatic patients worldwide. HDM is an immunologically complex antigen which activates both innate and adaptive immune responses (1). Moreover it was reported that proteinases, such as trypsin-like enzymes, in HDM promoted allergenicity to proteins, resulting in allergic reactions (2). To facilitate allergic asthma research, Chondrex, Inc. provides HDM extract as a clinically relevant allergen for the induction of a mouse asthma model that mimics how asthmatic patients are sensitized with aeroallergens.
General Protocols for Inducing Asthma in Balb/c Mice
1. Acute Asthma Model
![](https://cdn11.bigcommerce.com/s-ryt90hjx0j/product_images/uploaded_images/acute-asthma-model.png)
2. Chronic Asthma Model
![](https://cdn11.bigcommerce.com/s-ryt90hjx0j/product_images/uploaded_images/chronic-asthma-model.png)
Chronic Asthma Model
![](https://cdn11.bigcommerce.com/s-ryt90hjx0j/product_images/uploaded_images/a-chronic-asthma-model.png)
Six Balb/c mice received 150 µg HDM mixed with 0.2 mg Alum Adjuvant by weekly IP injections. Serum total IgE levels on weeks 4 and 7 were significantly higher than those of week 0 (blue). In addition, anti-HDM IgE levels were significantly higher on week 7 (orange) than they were on week 0 and 4.
Der p 1 Associated Trypsin Activity in HDM
![](https://cdn11.bigcommerce.com/s-ryt90hjx0j/product_images/uploaded_images/der-p-1-associated-trypsin-activity-in-hdm.png)
Chondrex, Inc. compared HDM from two vendors. Vendor A’s HDM showed high trypsin activities with cysteine activation, indicating that it contains high levels of cysteine proteinase, such as Der p 1. On the other hand, cysteine treatment did not activate Vendor B’s HDM, possibly due to interfering trypsin activities from other serine proteinases, such as Der p 3.