Oxytocin ELISA | RE52331
- SKU:
- 181-RE52331
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Description
Oxytocin ELISA | RE52331 | IBL International
Application: ELISA
Category Type: Neuropeptides; Pitui
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![IHC image of neurons staining for oxytocin in the rat hypothalamus and higher magnification of the rat hypothalamus with nickel preparation . The tissue was fixed with 4% formaldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. IHC image of neurons staining for oxytocin in the rat hypothalamus and higher magnification of the rat hypothalamus with nickel preparation . The tissue was fixed with 4% formaldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.](https://cdn11.bigcommerce.com/s-ryt90hjx0j/images/stencil/590x590/products/25081/31738/53__56969.1643454107.jpg?c=1)
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