Oxytocin ELISA | RE52331

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181-RE52331
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Oxytocin Antibody | 20068 IHC image of neurons staining for oxytocin in the rat hypothalamus and higher magnification of the rat hypothalamus with nickel preparation . The tissue was fixed with 4% formaldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
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