anti-CD152 | 359-020

Human CD152 (CTLA-4) is a cell surface glycoprotein expressed at low levels on activated T cells (1).  CD152  is a high affinity receptor for the costimulatory molecules CD80 (B7-1) and CD86 (B7-2) and appears to function as a negative regulator of T cell activation (2, 3).  

Isotype:  Murine IgG1kappa 

Immunogen:  Stimulated human PBL’s and human CD152 Ig fusion protein

Specificity:  Antibody ANC152.2/8H5 reacts with an extracellular epitope of human CTLA-4 expressed on activated T cells.  It has been reported to cross react with dog (11, 12), pig, cow and cat CD152.

 Functional Application:  ANC152.2 blocks binding of CD152 (CTLA-4) Ig fusion protein to its CD80/CD86 receptor, and has been reported to modulate suppressive function of human T Regulatory Cells in Vitro (4). ANC152.2 can bind to intracellular CD152 in fixed permeabolized cells.  Purified and  F(ab’)2 forms of this clone have been used to block  proliferation and/or IL-10 production by activated T cells in various models (5, 6, 7, 8, 9) .