Bioactive Sclerostin ELISA | BI-20472

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SKU:
BI-20472
Availability:
In Stock
Size:
1 kit
Packing:
96 tests
  • Bioactive Sclerostin ELISA | BI-20472
  • Bioactive Sclerostin ELISA | BI-20472
  • Bioactive Sclerostin ELISA | BI-20472
  • Bioactive Sclerostin ELISA | BI-20472
  • Bioactive Sclerostin ELISA | BI-20472
  • Bioactive Sclerostin ELISA | BI-20472
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Description

Bioactive Sclerostin ELISA | BI-20472

Bioactive Sclerostin ELISA Kit (Human SOST):

Bioactive Sclerostin ELISA Product Overview

The bioactive Sclerostin ELISA kit is a 3.5 hour, 96-well sandwich ELISA for the quantitative determination of bioactive Sclerostin in human serum, EDTA-plasma, and citrate plasma. The human Sclerostin ELISA kit employs human serum-based standards to ensure the measurement of biologically reliable data.

The bioactive Sclerostin ELISA kit uses highly purified, epitope mapped antibodies with characterized binding kinetics.

Bioactive Sclerostin ELISA Assay Principle

The Biomedica Sclerostin ELISA is a sandwich enzyme immunoassay for the quantitative determination of bioactive Sclerostin (SOST) in human serum and plasma samples.

The figure below explains the principle of the bioactive Sclerostin sandwich ELISA:


bioactive Sclerostin ELISA Assay Principle

Capture antibody: recombinant monoclonal anti-human Sclerostion antibody
Detection antibody: polyclonal goat anti-human Sclerostin antibody
Target antigen: human bioactive Sclerostin

In a first step, assay buffer is pipetted into the wells of the microtiter strips. Thereafter, standard/control/sample are pipetted into the wells, which are pre-coated with a recombinant monoclonal anti-human Sclerostin antibody. Any bioactive Sclerostin present in the standard/control/sample binds to the pre-coated antibody in the well. After incubation, a washing step is applied where all non-specific unbound material is removed. In a next step, the conjugate (anti-human Sclerostin-HRP) is pipetted into the wells and reacts with bioactive Sclerostin present in the sample, forming a sandwich. After another washing step, the substrate (tetramethylbenzidine; TMB) is pipetted into the wells. The enzyme-catalyzed color reaction of the substrate is directly proportional to the amount of bioactive sclerostin in the sample. This color change is detectable with a standard microtiter plate reader. A dose response curve of the absorbance (optical density, OD, at 450 nm) versus standard concentration is generated, using the values obtained from the standards. The concentration of bioactive Sclerostin in the sample is determined directly from the dose response curve.

Bioactive Sclerostin ELISA Typical Standard Curve

The figure below shows a typical standard curve for the bioactive human Sclerostin ELISA. The immunoassay is calibrated against recombinant human bioactive Sclerostin.


bioactive Sclerostin ELISA Typical standard curve

Bioactive Sclerostin ELISA Kit Components

Storage instructions: all reagents of the bioactive Sclerostin ELISA kit are stable at 4°C until the expiry date stated on the label of each reagent.

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