Cortical Glutamatergic Neurons | BX-0300

CONTENTS
• One vial of 5 million cryopreserved human neurons (500 μL)
• Neuron Seeding Supplement at 1000X
• Neuron Day 4 Supplement at 1000X
• Supplement K at 1000X
Immediately transfer the vial of neurons to liquid nitrogen upon receipt. Transfer the vials of supplements to a -20°C freezer. The supplements can be stored at -20°C for up to 6 months. Alternatively, the supplements can be stored at -80°C for up to 18 months.

ADDITIONAL MATERIALS NEEDED
• DMEM/F12 Medium (Life Technologies #11330-032)
• Neurobasal Medium (Life Technologies #21103-049)
• B27 Supplement (Life Technologies #17504-044)
• N2 Supplement (Life Technologies #17502-048)
• GlutaMAX (Life Technologies #35050-061)
• Geltrex (Life Technologies #A1413201)
• BDNF (Peprotech #450-02)
• GDNF (Peprotech #450-10)
• TGF-β1 (Peprotech #100-21C)
• PDL-Coated 96-Well Plates