Storage                      Shelf Life

4°C                              1 year

Protocoll

Add enough stain to cover gel completely. Incubate at room temperature according to the times listed below for lowest detectable level in a protein band. Gently agitate if possible.

1) 1 ug or more 2 minutes

2) 100 ng to 1 ug 10 minutes

3) 10ng to 100ng 60 minutes

It is possible to decrease these times by inserting gel and stain into microwave for 20 seconds.

For maximum sensitivity follow staining by a 20 minute gentle agitation in dionized water.

Protocoll for Mass Spectrometry

1) Cut out protein band and place in microfuge tube

2) Add 1 ml 30% ethanol or 30% acetone (30% acetic acid may be used but will result in acetylation of N-terminus).

3) Incubate for 30 minutes.

4) Repeat steps 2 and 3 until all stain removed from protein (typically 2 or 3 washes are required for complete stain removal)

5) Process as typical for mass spec analysis.