One-Step Blue® Protein Gel Stain is a ready-to-use visible blue protein gel staining solution. With no fixation or wash steps required, One-Step Blue® is a great choice for convenience and safety. One-Step Blue® can detect as little as approximately 10-20 ng of protein per band.

• One-step staining procedure, no fixation required
• Non-toxic formulation is safe for easy disposal
• Sensitive protein detection down to 10-20 ng
• Visualize blue bands, or image with a near-infrared fluorescence imager
• 1 L size
• Store at 4°C

Fast and simple procedure

One-Step Blue® produces fast (5-60 min) protein staining in a single step without fixation or washing. Destaining is optional. One-Step Blue® has comparable sensitivity as the widely used Coomassie Blue, with a lower limit of detection around 10-20 ng depending on the detection method used. Note that staining intensity varies between proteins. The staining is fully compatible with mass spectrometry and Edman-based sequencing. Proteins can be detected by visible blue staining, or by near-infrared fluorescence.

Results are clear. One-Step Blue® is a superior alternative to Coomassie and other colorimetric protein gel stains.

In a comparison experiment with Coomassie Brilliant Blue R-250 and Simply Blue™ SafeStain, One-Step Blue® demonstrated much clearer staining after 1 hour of destaining in water compared to other stains.