Osteoprotegerin ELISA | BI-20403

OPG ELISA highlights:

• Day test
• Most referenced human OPG ELISA

OPG ELISA Product Overview

The human Osteoprotegerin ELISA kit (OPG) is a 4.5 hour, 96-well sandwich ELISA for the quantitative determination of OPG in human serum and plasma. The Osteoprotegerin kit employs human serum-based standards to ensure the measurement of biologically reliable data.

OPG ELISA Assay Principle

The human OPG ELISA kit is a sandwich enzyme immunoassay for the determination of OPG in human serum and plasma samples.

The figure below explains the principle of the Osteoprotegerin sandwich ELISA:

Capture antibody: polyclonal goat anti-human OPG antibody
Detection antibody: monoclonal mouse anti-human OPG, biotin-labeled
Target antigen: human OPG

In a first step, assay buffer, standard/control/sample and detection antibody (biotinylated monoclonal mouse anti-human OPG) are pipetted into the wells of the microtiter strips, which are pre-coated with polyclonal goat anti-human OPG antibody. OPG present in the standard/control/sample binds to the pre-coated antibody in the well and forms a sandwich with the detection antibody. In the washing step all non-specific unbound material is removed. In a second step, the conjugate (streptavidin-HRP) is pipetted into the wells and reacts with the detection antibody. After another washing step, the substrate (TMB, tetramethylbenzidine) is pipetted into the wells. The enzyme-catalyzed color change of the substrate is directly proportional to the amount of OPG. This color change is detectable with a standard microtiter plate reader. A dose response curve of the absorbance (optical density, OD at 450 nm) vs. standard concentration is generated. The concentration of OPG in the sample is determined directly from the dose response curve.

OPG ELISA Typical Standard Curve

The figure below shows a typical standard curve for the ELISA for Osteoprotegerin. The immunoassay is calibrated against recombinant human OPG:

OPG ELISA ELISA Kit Components