Chicken IgA(Immunoglobulin A) ELISA Kit | ECH0083

Chicken IgA(Immunoglobulin A) ELISA Kit | ECH0083

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-
well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin
conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was
added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic
reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution.
The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in
a microplate reader, and then the concentration of target can be calculated.

Typical Data & Standard Curve

Results of a typical standard operation of a IgA ELISA Kit are listed below. This standard curve was generated
at our lab for demonstration purpose only. Users shall obtain standard curve as per experiment by
themselves. (N/A=not applicable) 

Specificity
This assay has high sensitivity and excellent specificity for detection of IgA. No significant cross-reactivity or
interference between IgA and analogues was observed.
Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection
between IgA and all the analogues, therefore, cross reaction may still exist.

Recovery
Matrices listed below were spiked with certain level of IgA and the recovery rates were calculated by
comparing the measured value to the expected amount of IgA in samples.

Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IgA and their
serial dilutions. The results were demonstrated by percentage of calculated concentration to the expectation.

Precision
Intra-Assay: CV<8%
Inter-Assay: CV<10%

Stability
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 10% within the expiration date under appropriate storage condition.