LAG-3 Antibody [1C6] | SD8842

LAG-3 Antibody [1C6]

Specifications

• HOST SPECIES: Llama
• SPECIES REACTIVITY: Human
• IMMUNOGEN: LAG3 antibody was raised against a recombinant protein corresponding to amino acids 23-434 of human LAG3.
• CONJUGATE: Unconjugated
• TESTED APPLICATIONS: E, Flow, IF, IHC-P
• APPLICATION NOTE: LAG-3 antibody can be used for ELISA starting at 0.1 μg/mL. For immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 5 μg/mL. For flow cytometry at 1 μg/ml.

Properties

• PURIFICATION: LAG3 Antibody is affinity chromatography purified via Nickel column. Antibody is supplied as a His-tagged purified protein.It also contains a myc-tag for detection.
• CLONALITY: Recombinant Monoclonal
• ISOTYPE: sdAb
• PHYSICAL STATE: Liquid
• BUFFER: LAG-3 Antibody is supplied in PBS.
• CONCENTRATION: 1 mg/mL
• STORAGE CONDITIONS: LAG3 antibody should be stored in working aliquots at -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

• NCBI OFFICIAL SYMBOL: LAG3
• ADDITIONAL NAMES: LAG3 Antibody: lymphocyte activating 3, LAG-3, CD223
• Protein Accession Number: NP_002277
• PROTEIN GI NUMBER: 167614500
• NCBI GENE ID NUMBER: 3902
• USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background

• The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4). LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy.
• 1: Huard B, Tournier M, Hercend T, et al. Lymphocyte-activation gene 3/major histocompatibility complex class II interaction modulates the antigenic response of CD4+ T lymphocytes. Eur J Immunol 1994; 24:321621.
• 2: Triebel F. LAG-3: a regulator of T-cell and DC responses and its use in therapeutic vaccination. Trends Immunol 2003; 24:61922.
• 3: Workman CJ, Wang Y, El Kasmi KC, et al. LAG-3 regulates plasmacytoid dendritic cell homeostasis. J Immunol. 2009; 182:188591.
• 4: Workman CJ, Vignali DA. The CD4-related molecule, LAG-3 (CD223), regulates the expansion of activated T cells. Eur J Immunol 2003; 33:9709.