This product leads to effective elution of antibodies from protein A columns under mild pH (pH 4.0). Normally, antibodies are not effectively eluted at pH 4.0. Use of a lower pH can lead to partial denaturation and subsequent aggregation of the eluted antibodies. This product is based on the unique characteristics of arginine, which suppresses protein-protein interactions.

This product is manufactured with permission from Ajinomoto Co., Inc. based on the patent JP 4826995*.

*JP: 4826995, US: 8084032, 8435527, 2012-0264918, EP: 1568710, CN: 1680426

Features

• Enables effective elution of antibodies from protein A column, reducing potential risk of acid denaturation and resultant aggregation
• Virus inactivation, a key step of clinical antibody manufacturing, is enhanced by arginine
• The use of this product has no impact on the preceding processes of antibody purification, e.g., loading step of cell culture medium and the following column washing step
• Arginine-based elution buffers are available in different formats upon request

Elution from Protein A using L-Arginine

Applications