Zymolyase®-20T (100T)

Zymolyase®-20T | 07663-91 from Nacalai Tesque is available for delivery.

Zymolyase^®, produced by a submerged culture of Arthrobacter luteus⁽¹⁾, has strong lytic activity against living yeast cell walls^(2),(3) to produce protoplast or spheroplast of various strains of yeast cells. An essential enzyme for the lytic activity of Zymolyase^® is β-1,3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with β-1,3-linkages and releases specifically laminaripentaose as the main and minimum product unit.

Product Information

There are two preparations of Zymolyase^®, Zymolyase^®-20T and Zymolyase^®-100T, having lytic activity of 20,000 units/g and 100,000 units/g respectively. Zymolyase^®-20T is ammonium sulfate precipitate while Zymolyase^®-100T is a further purified preparation by affinity chromatography⁽⁹⁾. Lytic activity varies depending on yeast strain, growth stage of yeast, or cultural conditions^(6-8). Further informations related to Zymolyase^® can be obtained in the reference section below

Unit Definition

One unit of lytic activity is defined as that amount which indicates 30% of decrease in absorbance at 800 nm(A₈₀₀) of the reaction mixture under the following condition.

Applications