nNOS:N-Terminal (neuronal Nitric Oxide Synthase) Antibody | 24431

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SKU:
484-24431
Quantity/Volume:
100 µL
  • nNOS:N-Terminal (neuronal Nitric Oxide Synthase) Antibody | 24431
  • IHC image of neurons staining for the N-terminal of nNOS in the rat cortex and the rat striatum. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:2000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen, bottom left image with nickel stain.
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Description

nNOS:N-Terminal (neuronal Nitric Oxide Synthase) Antibody | 24431 | ImmunoStar

The ImmunoStar N-terminal neuronal nitric oxide synthase antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus, striatum, cortex and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/1,000 – 1/2,000 in PBS/0.3% Triton X-100 – biotin/avidin-HRP. By Western blot analysis of brain homogenates the antibody specifically labels a band of approximately 155 kD. Immunolabeling is completely abolished by pre-adsorption with synthetic human nNOS (134-148) at 5 µg per mL of diluted antibody. No cross reactivity with other forms of NOS was observed.

Host: Rabbit

State: Lyophilized Whole Serum

Reacts With: Mouse, Rat

Availability: In Stock

Alternate Names: Constitutive NOS; NC-NOS; NOS type I; Neuronal NOS; Nitric Oxide Synthase; bNOS; Nitric oxide synthase-brain; N-NOS; Peptidyl-cysteine S-nitrosylase; IHPS1; NC-NOS; nitric oxide synthase 1 (neuronal), anti-nNOS:N-Terminal

Gene Symbol / ID, Accession #: NOS1, 4842

RRID: AB_572255

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