Proteinase K solution 10ml (20mg/ml) | 502-PK

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SKU:
502-PK
Size:
10 mL
Select Currency //300.00

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Description

Proteinase K is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic or hydrophobic amino acids. Proteinase K is classified as a serine protease. The smallest peptide to be hydrolysed is a tetrapeptide.


DNA Extraction lysis reagent for DNA tests & genotyping: The Proteinase K solution is useful when a small amount of biological samples are processed. Add the enzyme solution to DirectPCR reagents at the final concentration of 0.5-1.0 mg/ml (25-50 μl Proteinase K solution per 1 ml DirectPCR reagent). Follow the instruction manuals for DirectPCR Reagent Protocol/Manual for each blood, blood stain, buccal swab, hair root DNA genotyping. Once diluted in DirectPCR reagents, Proteinase K is stable for ~24 hrs.


Other applications: Proteinase K digests native proteins very effectively. It can therefore be used to remove DNases and RNases when isolating DNA and RNA from tissues or cells.


Usage Conditions:

• Proteinase K is active over a wide pH range—optimal activity between 6.5 and 9.5

• Under denaturing conditions—e.g., in the presence of SDS or urea

• In the presence of metal chelating agents—e.g., EDTA

• At high temperatures—optimum digestion temperature is 65°C


Unit Definition: One unit of the enzyme liberates folin-positive amino acids and peptides corresponding to 1μmol tyrosine in 1min at 37°C using denatured hemoglobin as substrate.


Quality Control: Tested for the absence of endo-, exodeoxyribonucleases and ribonucleases

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3 Reviews

  • 5

    producto increíble

    Posted by Gustavo on 24th Jun 2022

    Es un producto increíble, lo he usado y los resultados que he visto son bastante buenos.

  • 5

    amazing product

    Posted by Colton on 24th Jun 2022

    This product is amazing i have use this and this product have very affective results.

  • 5

    superb packing

    Posted by Waylon on 6th Jan 2022

    the way they packed their products is just awesome and professional. Really impressed

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