Recombinant Protein A/G (r-PAG)
- SKU:
- GEN-NRPA14S
- Formulation:
- lyophilized from 5 mM of PB (4 mM of Na2HPO4, 1 mM of NaH2PO4, pH 7.4)
- Mol. Weight:
- 56.3 kDa
- Theoretical pI:
- 4.74
- Theoretical activity:
- 18.1 U / mg protein (rc-SA: Biotin = 1: 4 (mol: mol))
- Resources:
- Escherichia coli (E. coli)
- Purity:
- ≥95% by SDS-PAGE analysis
- Absorption coefficient:
- 280nm absorption method, the molar absorption coefficient is 3.74 × 104 (mol / L) -1cm-1 ， c (g / L) = A280nm / 0.66
- Endotoxin:
- ≤5EU / mg
- Storage condition:
- -20°C
- Storage period:
- 3 years
Description
The recombinant protein a G protein fusion protein (r-pag) fused 8 Fc binding regions of the antibody, including 5 Fc binding domains from Staphylococcus aureus protein A and 3 Fc binding domains from G protein from Staphylococcus aureus. Streptococcus, and eliminated the cell wall binding domain, albumin binding domain and non-specific binding domain of protein A and protein G. Compared to a single protein A or protein G, it has a range broader binding and can bind to all human IgG subtypes, IgA, IgE, and IgM, but not mouse IgA, IgM, and serum albumin. It is suitable for the extraction and detection of mouse IgG monoclonal antibody. At the same time, the fused r-pag reduced pH dependence and allowed binding to pH5-8.
M ： Standard protein marker
Track 1 ： r-PAG
For research only!
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