Mouse Anti S-AdenosylHomocysteine (SAH) Clone 301-10 | MA00302-50

Mouse Anti S-AdenosylHomocysteine (SAH) Clone 301-10 | MA00302-50 | Arthus Biosystems

Product name         

Mouse anti-SAH 2a

Catalog Number     

MA00302-50

Description              

Mouse monoclonal antibody against S-Adenosylhomocysteine [301-10]

Specificity                 

MA00302 shows the following reactivities with related compounds: S-Adenosylhomocysteine: 100%, S-Adenosylmethionine: -1.5%, Adenosine: <1 %, Homocysteine: < 1%, L-Cysteine: < 1%, Glutathione: < 1%, L-Cystathionine: <   1%, Methythioadenosine (MTA): < 5%, ADP (adenosine diphosphate): < 1%, ATP (adenosine triphosphate): < 1%.

Immunogen              

S-Adenosylhomocysteine conjugated to BSA

Properties

Form                                   

Liquid

Storage instructions          

Store at 4°C, -20°C for long term storage

Storage buffer                   

PBS 10mM pH7.4 (NaCI 150mM), Sodium azide 0.02%, BSA 10mg/m1 or PBS 10mM, pH7.4 (NaCI 150mM), Sodium azide 0.02%, Glycerol 50%, BSA 10mg/mi

Purity                                 

>95% Purified from mouse ascites fluid by affinity chromatography

Clonality                             

Monoclonal

Clone number                   

301-10

Immunoglobin isotype      

IgG3

Affinity                               

Ka = 4.20 x 108L/mol ( 1.93 x 10-8M )

Research Areas                  

• Methylation of biomolecules (DNA, RNA, proteins, hormones, neurotransmitters, etc.)
• One-carbon metabolism
• Signal Transduction
• Metabolism
• Pathways and Processes Cancers
• Arthritis
• Heart diseases
• Neurodegenerative diseases
• Atherosclerosis
• Liver diseases
• Kidney diseases

Applications

The use of MA00302 in the following tested applications has been tested. The application notes include recommended starting dilutions. Optimal dilutions/concentrations should be determined by the end user. Higher dilution than suggested maybe used in IHC and IF. The product may be used in other not-yet-tested applications.

Notes

• cELISA:  1:2000/4000
• FCM :   1:100
• IHC :   1:100

Target

S-adenosylhomocysteine is a competitive inhibitor of S-adenosylmethionine-dependant methyl transferase reactions. Therefore, it plays a key role in the control of methylation via regulation of the intracellular concentration of S-adenosylhomocysteine.

Cellular localization

Cytoplasm, nuclear

Figure 1: Immunohistochemistry staining was performed using MA00302 with benign breast tissue adjacent to breast cancer. Brown areas indicated positive staining in nuclear and cytoplasmic areas (x400).

Figure 2: Control for FCM. Normal liver cells L02 and carcinoma cells Hep G2 were stained with the buffer without any antibody.

Figure 3: FCM results from normal liver cell line L02 and hepatocyte carcinoma  cell  line  Hep  G2  stained  with  anti-SAH  monoclonal antibody from clone 301-10. Average fluorescence signal in Hep G2 cells (56.99) was reduced compared to that in L02 cells (103.36), indicating SAM level is reduced during carcinogenesis.