Mouse Anti S-AdenosylHomocysteine (SAH) Clone 844-1 | MA00305

Mouse Anti S-AdenosylHomocysteine (SAH) Clone 844-1 | MA00305 | Arthus Biosystems

Product name         

Mouse anti-SAH 5a/b

Catalog Number     

MA00305-50/100

Description              

Mouse monoclonal antibody against S-Adenosylhomocysteine [844-1]

Specificity                 

MA00305 shows the following reactivities with related compounds: S-Adenosylhomocysteine: 100%, S-Adenosylmethionine: -2.6%, Adenosine: < 1%, Homocysteine: < 1%, L-Cysteine: < 1%, Glutathione: < 1%, L-Cystathionine: <   1%, Methythioadenosine (MTA): < 5%, ADP (adenosine diphosphate): < 1%, ATP (adenosine triphosphate): < 1%.

Immunogen              

S-Adenosylhomocysteine conjugated to BSA

Properties

Form                                   

Liquid

Storage instructions          

Store at 4°C, -20°C for long term storage

Storage buffer                    

PBS 10mM pH7.4 (NaCI 150mM), Sodium azide 0.02%, BSA 10mg/m1 or PBS 10mM, pH7.4 (NaCI 150mM), Sodium azide 0.02%, Glycerol 50%, BSA 10mg/m1

Purity                                 

>95% Purified from mouse ascites fluid by affinity chromatography

Clonality                             

Monoclonal

Clone number                    

844-1

Immunoglobin isotype      

IgG2a

Affinity                               

Ka = 7.05 x 109L/mol( 1.42 x 10-19M   )

Research Areas                  

• Methylation of biomolecules (DNA, RNA, proteins, hormones, neurotransmitters, etc.)
• One-carbon metabolism
• Signal Transduction
• Metabolism
• Pathways and Processes Cancers
• Arthritis
• Heart diseases
• Neurodegenerative diseases Atherosclerosis
• Liver diseases
• Kidney diseases

Applications

The use of MA00305 in the following tested applications has been tested. The application notes include recommended starting dilutions. Optimal dilutions/concentrations should be determined by the end user. Higher dilution than suggested maybe used in IHC and IF. The product may be used in other not-yet-tested applications.

Notes

• cELISA:  1:30,000/36,000
• FCM:   1:300
• IHC:  1:300

Target

S-adenosylhomocysteine is a competitive inhibitor of S-adenosylmethionine-dependant methyl transferase reactions. Therefore, it plays a key role in the control of methylation via regulation of the intracellular concentration of S-adenosylhomocysteine.

Cellular localization

Cytoplasm, nuclear

Figure 1: Immunohistochemistry staining was performed using MA00305 with benign liver tissue adjacent to liver cancer. Brown areas indicated strong positive staining in nuclear and cytoplasmic areas (x400).