Mouse Anti S-AdenosylHomocysteine (SAH) Clone 301-16 | MA00304-50

Mouse Anti S-AdenosylHomocysteine (SAH) Clone 301-16 | MA00304-50 | Arthus Biosystems

Product name         

Mouse anti-SAH 4a

Catalog Number     

MA00304-50

Description              

Mouse monoclonal antibody against S-Adenosylhomocysteine [301-16]

Specificity               

MA00304 shows the following reactivities with related compounds: S-Adenosylhomocysteine: 100%, S-Adenosylmethionine: < 3%, Adenosine: < 1%, Homocysteine: < 1%, L-Cysteine: < 1%, Glutathione: < 1%, L-Cystathionine: <   1%, Methythioadenosine (MTA): < 5%, ADP (adenosine diphosphate): < 1%, ATP (adenosine triphosphate): < 1%

Immunogen              

S-Adenosylhomocysteine conjugated to BSA

Properties

Form                                   

Liquid

Storage instructions          

Store at 4°C, -20°C for long term storage

Storage buffer                    

PBS 10mM pH7.4 (NaCI 150mM), Sodium azide 0.02%, BSA 10mg/m1 or PBS 10mM, pH7.4 (NaC1150mM), Sodium azide 0.02%, Glycerol 50%, BSA 10mg/m1

Purity                                 

>95% Purified from mouse ascites fluid by affinity chromatography

Clonality                             

Monoclonal

Clone number                    

301-16

Immunoglobin isotype      

IgG3

Affinity                               

Ka = 6.13 x 108L/mol (1.63 x 10-8M)

Research Areas                  

• Methylation of biomolecules (DNA, RNA, proteins, hormones, neurotransmitters, etc.)
• One-carbon metabolism
• Signal Transduction
• Metabolism
• Pathways and Processes Cancers
• Arthritis
• Heart diseases
• Neurodegenerative diseases Atherosclerosis
• Liver diseases
• Kidney diseases

Applications

The use of MA00304 in the following tested applications has been tested. The application notes include recommended starting dilutions. Optimal dilutions/concentrations should be determined by the end user. Higher dilution than suggested maybe used in IHC and IF. The product may be used in other not-yet-tested applications.

Notes

• cELISA:   1:2000/4000
• FCM:   1:100
• IHC:   1:100

Target

S-adenosylhomocysteine is a competitive inhibitor of S-adenosylmethionine-dependant methyl transferase reactions. Therefore, it plays a key role in the control of methylation via regulation of the intracellular concentration of S-adenosylhomocysteine.

Cellular localization

Cytoplasm, nuclear

Figure 1: Competitive ELISA using anti-S-Adenosymethionine monoclonal antibody [301-16]

The 0.5 pg/ml of SAH-BSA was coated into 96 wells. Serial dilution of SAH standard (SAHNa), S-Adenosylmethionine SAM from Sigma-Aldrich Cat# A2408), Homocysteine (H-Cys), L-Cysteine (L-Cys), Adenosine (Ade), Glutathione (GST),  L-  Cystathionine  (L-CTT), Methythioadenosine (MTA),  ADP (adenosine  diphosphate), ATP (adenosine triphosphate) and  properly diluted  MA00304 were added. HRP conjugated Goat anti-Mouse IgG antibody was used to develop the color. OD450 value was measured on each well.

Figure 2: Immunohistochemistry staining was performed using MA00304 with benign  liver tissue adjacent to carcinoma.   Brown areas indicated strong positive staining in nuclear and cytoplasmic areas(x400).

Figure 3: The same samples as in Figure 2 from liver cancer area. Cytoplasmic and nuclear areas showed negative and some background staining (probably extra antibody was used) (x400).