Rabbit Polyclonal Antiserum Methionine AdenosylTransferase | PA00401-50

Rabbit Polyclonal Antiserum Methionine AdenosylTransferase | PA00401-50 | Arthus Biosystems

Product name       

Rabbit anti-MAT s, Rabbit anti-MAT g

Catalog Number  

PA00401-50 (no glycerol), PA00402-50 (with glycerol)

Description             

Rabbit polyclonal antiserum against methionine adenosyltransferase (MAT, E.C. 2.5.1.6)

Properties

Form                                                     

Liquid

Storage instruction             

Store at 4°C if used within 1-2 weeks, store at below -20°C for long term

Storage buffer                     

Antiserum in PBS 10mM pH 7.4 (NaCI 150mM), sodium azide 0.02% (glycerol 50%)

Packaging                           

50 pl

Clonality                             

Rabbit polyclonal

Titer                               

— 1:420,000 (ELISA)

Specificity                      

SAM: S-adenosylmethionine, SAH: S-adenosylhomocysteine, BSA: Bovine Serum Albumin

Immunogen                        

recombinant MAT conjugated to KLH

Research Areas

• Methylation of biomolecules (DNA, RNA, proteins, hormones, neurotransmitters, etc.)
• One-carbon and mitochondria! metabolisms
• Signal transduction
• Epigenetics
• Pathways and processes
• Cancer and other disorders

Applications

The use of PA00401 or Pa00402 in the following applications has been tested. The application notes include recommended starting dilutions. Optimal dilutions/concentrations should be determined by the end user. The product can be used in other applications such as IHC, Immunoprecipitation etc.

Notes

ELISA:  1:-120,000

Target

Except for parasites that rely on host for living, cells from all organisms have methionine adenyltransferase (MAT, EC2.5.1.6), also known as S-adenosylmethionine synthetase.   MAT genes have been found to be exceptionally conserved throughout evolution. It was reported that there is 59% homology between human and E. coli MAT gene sequences. In mammals, three forms or isozymes of MAT have been identified that are encoded by three MAT genes. The MAT1a gene encodes al catalytic subunit. MAT-I is a tetramer of al subunits and MAT-III a dimer of the same subunits. Both MAT-I and MAT-III are present in adult liver cells. MAT-II is a heterotetramer formed by MAT2a encoding the catalytic subunit of a2 and MAT2b gene encoding regulatory 6 subunit, present in cells other than liver, embryonic liver and hepatoma cells. MAT catalytic reaction in the body is divided into two steps:

(1) Catalyze L- methionine (L-Met) and adenosine triphosphate (ATP) to generate S-adenosylmethionine (known as the active methionine, SAM) and tripolyphosphate (PPPi). Both SAM and PPPi remain on the surface of MAT at this stage.

(2)The phosphatase activity of MAT further decompose  PPPi to dimeric phosphoric acid (PPi) and inorganic monophosphate (Pi).  SAM  can only be synthesized  by MAT.  SAM  is one of the few sulfur-containing active substances that carry extre mely diverse and important biological functions in nature and is the key molecules in the methionine cycle.

Cellular localization:  cytoplasm, nucleus