Biotin 10C & 2N aza-S-AdenosylMethionine (SAM) | ACT00206

Biotin 10C & 2N aza-S-AdenosylMethionine (SAM) | ACT00206 | Arthus Biosystems

Product name       

Bio-12CN-aza-SAM 1a/b/

Catalog Number  

ACT00206-10/50/100

Description            

Biotin is conjugated with aza-SAM through a 10-carbon and 2-nitrogen linker.

Properties

Form 

Liquid

Molecular formula

C35H54N1007S

Molecular Weight

758.93

Storage instructions Storage buffer

Store at -20°C

Storage Buffer

PB 20mM, pH 7.4, Proclin 0.1%

Concentration

0.5 mg/m

lPurity

100% (HPLC)

Structure

Verification
The ninhydrin colorimetry and thin layer chromatography methods were used to show reactions were complete and the conjugated products have the Aza-SAM component. Thorough purification was performed in each step to ensure removal of any non-conjugated materials. Ultraviolet absorption spectrum of the
conjugated product showed a peak at 255.5nm. Aza-SAM UV absorption spectrum does not show any peak.

Applications

The use of ACT00206-10/50/100 in the following application has been tested. Optimal concentrations should be determined by the end user. The product may be used in other not-yet-tested applications.

Competitive ELISA:

(Plates coated with PLL—aza—SAM, SA,Mouse—anti—SAM antibody respectively)

Notes

NO DIFFERENCE   BETWEEN   BIOTIN CONJUGATED AND UNCONJUGATED SAM MOLECULES IN THEIR CAPABILITIES TO COMPITIVELY BIND ANTI-SAM ANTIBODY

Figure 1: Streptavidin was coated on micro-plate, followed by Bio-12CN-aza-SAM. HRP-anti-SAM antibody was then added and OD450 was read.