Biotin 10C & 2N aza-S-AdenosylMethionine (SAM) | ACT00206
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Biotin 10C & 2N aza-S-AdenosylMethionine (SAM) | ACT00206 | Arthus Biosystems
Biotin is conjugated with aza-SAM through a 10-carbon and 2-nitrogen linker.
Storage instructions Storage buffer
Store at -20°C
PB 20mM, pH 7.4, Proclin 0.1%
The ninhydrin colorimetry and thin layer chromatography methods were used to show reactions were complete and the conjugated products have the Aza-SAM component. Thorough purification was performed in each step to ensure removal of any non-conjugated materials. Ultraviolet absorption spectrum of the
conjugated product showed a peak at 255.5nm. Aza-SAM UV absorption spectrum does not show any peak.
The use of ACT00206-10/50/100 in the following application has been tested. Optimal concentrations should be determined by the end user. The product may be used in other not-yet-tested applications.
(Plates coated with PLL—aza—SAM, SA,Mouse—anti—SAM antibody respectively)
NO DIFFERENCE BETWEEN BIOTIN CONJUGATED AND UNCONJUGATED SAM MOLECULES IN THEIR CAPABILITIES TO COMPITIVELY BIND ANTI-SAM ANTIBODY
Figure 1: Streptavidin was coated on micro-plate, followed by Bio-12CN-aza-SAM. HRP-anti-SAM antibody was then added and OD450 was read.