Biotin 6C aza-S-AdenosylMethionine (SAM) | ACT00205

Biotin 6C aza-S-AdenosylMethionine (SAM) | ACT00205 | Arthus Biosystems

Product name       

Bio-6C-aza-SAM 1a/b/c

Catalog Number

ACT00205-10/50/100

Description         

Biotin-conjugated aza-SAM: Biotin is conjugated to the NH2 of aza-SAM through 6-aminohexanoic acid.

Properties

Form                                   

Liquid

Molecular formula              

C31 H48' N908S

Molecular Weight               

742.29

Storage instructions          

Store at -20°C

Storage buffer                    

PB 20mM pH 7.4, Proclin 0.1%

Concentration                    

0.5 mg/ml

Purity                                  

100% (HPLC)

Structure

Verification

The   ninhydrin   colorimetry  and  thin   layer chromatography  methods  were  used  to  show reactions  were  complete  and  the  conjugated products   have  the  Aza-SAM  component. Thorough  purification  was  performed  in  each step to ensure removal of any non-conjugated materials.Ultraviolet absorption spectrum of the conjugated product showed a peak at 255.5nm. Aza-SAM UV absorption spectrum does not show any peak.

Applications

The use of ACT00205-10/50/100 in the following application has been tested. Optimal concentrations should be determined by the end user. The product may be used in other not-yet-tested applications.

Competitive ELISA

(Plates coated with PLL—aza—SAM, SA,Mouse—anti—SAM antibody respectively)

Notes

NO DIFFERENCE   BETWEEN   BIOTIN CONJUGATED AND UNCONJUGATED SAM MOLECULES IN THEIR CAPABILITIES TO COMPITIVELY BIND ANTI-SAM ANTIBODY.

Figure 1: Streptavidin was coated on micro-plate, followed by Bio-6C-aza--SAM. HRP-anti-SAM antibody was then added and OD450 was read.