Transforming Growth Factor B (TGF-β) is a stable, multifunctional polypeptide growth factor. TGF-β exists in at least five unique isoforms; TGF-β1, TGF-β2, TGF-β3, TGF-β4, and TGF-946;5. TGF-946;1 is the prevalent form and is found most ubiquitously while the other isoforms are expressed in a more limited spectrum of cells and tissues. It is normally secreted as an inactive or latent complex.

The Fast Human TGF-β1 ELISA is a solid phase ELISA designed to measure human TGF-β1 levels in cell culture supernatants, serum, and plasma. The main feature of this kit is its use of a novel fast approach to replace traditional methods. The kit is designed to ensure short steps, a short incubation time, and only one-set washing out. The measurement can be finished within 2 hours instead of the traditional 4-5 hours. The detection range is between 10-4,000 pg/mL. The levels of human TGF-β1 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human TGF-β1 protein.

Principle of the Assay:

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for human TGF-β1 was pre-coated onto a microplate. Standards and samples are then pipetted into the wells and incubated with a detection antibody specific for human TGF-β1. Aspirate each well and add Streptavidin-HRP, incubate the complex. Following a wash to remove any unbound antibodies and samples, an ultra-sensitive TMB substrate solution is added to the wells for the development of color. The color intensity is proportional to the amount of TGF-β1 bound in the initial step and can be measured by a plate reader at λ = 450nm.

Kit Contents and Storage Conditions: