Thidiazuron (TDZ) Solution

Thidiazuron (TDZ) is a plant growth regulator and a synthetic diphenylurea cytokinin widely used in plant tissue culture applications to promote shoot regeneration, organogenesis, and somatic embryogenesis.

The Benefits of Thidiazuron (TDZ)

• Easy to use
• Effective in stimulating shoot multiplication from explants (tissue samples) in micropropagation
• Used to supplement tissue culture media, such as MS media.
• Help overcome apical dominance
• Crucial for the micropropagation of some plants. 

How To Use Thidiazuron (TDZ) Solution

Follow the given steps to prepare the tissue culture media using PCT’s supreme grade agar:

1. Prepare liquid tissue culture media using PCT’s MS or DKW media, or any other alternative media based on your plant. 
2. Add sugar based on the quantity of media you’re making (generally for 1-liter media 30 grams of sugar is added), 1 ml/L PPM, and 3-4 grams per liter of gellan gum or 6-8 grams per liter of agar.
3. Then, add the desired quantity of the plant hormone.
4. Autoclave the medium for 20 minutes at 15 psi and 121℃.