Description
Thidiazuron (TDZ) is a plant growth regulator and a synthetic diphenylurea cytokinin widely used in plant tissue culture applications to promote shoot regeneration, organogenesis, and somatic embryogenesis.
The Benefits of Thidiazuron (TDZ)
- Easy to use
- Effective in stimulating shoot multiplication from explants (tissue samples) in micropropagation
- Used to supplement tissue culture media, such as MS media.
- Help overcome apical dominance
- Crucial for the micropropagation of some plants.
How To Use Thidiazuron (TDZ) Solution
Follow the given steps to prepare the tissue culture media using PCT’s supreme grade agar:
- Prepare liquid tissue culture media using PCT’s MS or DKW media, or any other alternative media based on your plant.
- Add sugar based on the quantity of media you’re making (generally for 1-liter media 30 grams of sugar is added), 1 ml/L PPM, and 3-4 grams per liter of gellan gum or 6-8 grams per liter of agar.
- Then, add the desired quantity of the plant hormone.
- Autoclave the medium for 20 minutes at 15 psi and 121℃.