abm | asCpf1 Nuclease NLS Protein

ABMGood | asCpf1 Nuclease NLS Protein | K188

Using Cpf1 (a.k.a. Cas12a) in your CRISPR experiment offers several advantages over other CRISPR-associated nucleases.

• Due to the T-rich PAM sequence (TTTN), Cpf1 enables editing in regions unable to be targeted by Cas9.
• Cpf1 can be used with a shorter guide RNA (called crRNA) than Cas9.
• Cpf1 creates a staggered cut in dsDNA instead of a blunt cut.
• Cpf1 cuts distal to the PAM sequence, which may allow for multiple rounds of cleavage.
asCpf1 is from the bacteria Acidaminococcus. This protein contains a SV40 T antigen nuclear localization signal (NLS) on the N-terminus of the protein. If the cut caused by asCpf1 is repaired by non-homologous end joining (NHEJ), an indel may be formed that disrupts the open reading frame of the targeted gene, leading to gene knockout. Alternatively, by supplying a repair template, a sequence can be knocked in at the cleavage site via homology directed repair (HDR).

Concentration:

10 μM, 1.5 mg/ml

Form:

Enzyme supplied with 10X Reaction Buffer.

Alternate Name:

Cas12a, asCas12a, Cpf1 from Acidaminococcus, Cas12a from Acidaminococcus

Reaction Buffer:

N/A

Reaction:

N/A

Notes:

This product is distributed for laboratory research only. Caution: Not for diagnostic use.

Application:

N/A

Components:

N/A

Inactivation:

N/A

Source:

E. coli

Shipping conditions:

Ice Packs

Storage conditions:

Store all components at -20°C.

Storag Buffer:

10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycerol.