abm | Immortalized Human Mammary Epithelial Cells (HMEC 2.6) | T0454

abm | Immortalized Human Mammary Epithelial Cells (HMEC 2.6) | T0454

Immortalization Method:

Serial passaging and transduction with LXSN-based retroviruses carrying hTERT and a mutant CDK4(R24C)

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Mammary Tissue

Organ Type:

Breast

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

Mean doubling time of around 72 hours.

Seeding Density:

10,000 - 15,000 cells/cm10²

Markers:

E-cadherin, EGFR, MIB1, CD10, CK5/14, P63, CK7/18, G418 resistance

Donor Age:

N/A

Donor Gender:

Female

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow IV . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 1% or 35 µg/ml bovine pituitary extract (Hammond), 12.5 ng EGF (Z100135), 50 µM fresh ascorbic acid, 2 nM estradiol, 1 µg/ml insulin, 2.8 µM hydrocortisone, 0.1 mM ethanolamine, 0.1 mM L-glutamine , 15 nM sodium selenite, and Penicillin/Streptomycin Solution to a final concentration of 1%. For selection use G418 at 100 µg/ml and Zeocin at 20 µg/ml. Adjust media pH to 7.4.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1) Cell markers were probed via immunohistochemical analysis; 2) Soft agar assay preformed to determine anchorage-independence.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C