abm | Immortalized Human Mammary Epithelial Cells | T0450

abm | Immortalized Human Mammary Epithelial Cells | T0450

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying SV40 gene.

BioSafety Level:

II

Organism:

Homo sapiens

Species:

Human

Source Organ:

Mammary Gland

Organ Type:

Breast

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

10,000 - 15,000 cells per cm10²

Markers:

CD10+, MUC1-, cytokeratin; note that these cells lack drug-resistance markers

Donor Age:

23

Donor Gender:

Female

Donor Ethnicity:

Caucasian

Propagation:

The base medium for this cell line is Prigrow IV medium available at abm. to a final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Real Time PCR was used to quantify the transgene expression.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C