abm | Immortalized Mouse Floxed Fam20c Osteoblast Cells | T0038
- SKU:
- T0038
- Availability:
- 5 to 7 Days Shipment
- Size:
- 1x10⁶ cells / 1.0 ml
Description
abm | Immortalized Mouse Floxed Fam20c Osteoblast Cells | T0038
Immortalization Method:
Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen
BioSafety Level:
II
Organism:
Postnatal 1 Day Fam20c f/f Mice
Species:
Mouse
Source Organ:
Calvarial bones
Organ Type:
Skeletal Muscle
Growth Properties:
Adherent
Morphology:
Polygonal
Passage Number:
N/A
Population Doubling:
N/A
Seeding Density:
30,000-40,000 cells/cm10²; Recommend split ratio is 1:2 to 1:4
Markers:
N/A
Donor Age:
N/A
Donor Gender:
N/A
Donor Ethnicity:
N/A
Propagation:
The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.
Quality Control:
Immunohistochemistry for gene expression associated with the differentiation and mineralization of osteoblasts
Shipping Condition:
Dry Ice
Storage Condition:
liquid nitrogen or -180C