abm | Immortalized Mouse Floxed Fam20c Osteoblast Cells | T0038

abm | Immortalized Mouse Floxed Fam20c Osteoblast Cells | T0038

in vitro for research on the field of mineralization disorders.

Immortalization Method:

Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen

BioSafety Level:

II

Organism:

Postnatal 1 Day Fam20c ^f/f Mice

Species:

Mouse

Source Organ:

Calvarial bones

Organ Type:

Skeletal Muscle

Growth Properties:

Adherent

Morphology:

Polygonal

Passage Number:

N/A

Population Doubling:

N/A

Seeding Density:

30,000-40,000 cells/cm10²; Recommend split ratio is 1:2 to 1:4

Markers:

N/A

Donor Age:

N/A

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

Immunohistochemistry for gene expression associated with the differentiation and mineralization of osteoblasts

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C