abm | Immortalized Rat Portal Myofibroblasts (RGF) | T0068

abm | Immortalized Rat Portal Myofibroblasts (RGF) | T0068

abm offers the sorted Immortalized Rat Portal Myofibroblasts (RGF-N2) (T0069).

Immortalization Method:

Serical passaging and chemical mediated transfection with SV40 large T Antigen mammalian expression vector

BioSafety Level:

II

Organism:

Sprague-Dawley Rat

Species:

Rat

Source Organ:

Liver

Organ Type:

Liver

Growth Properties:

Adherent

Morphology:

Spindle shaped

Passage Number:

N/A

Population Doubling:

13 - 23 hours

Seeding Density:

15,000 - 20,000 cells/cm10²

Markers:

alpha smooth muscle actin, type I collagen alpha-1, tissue inhibitor of metalloproteinases-1, PF-specific markers elastin, type XV collagen alpha-1, Ntpdase2/Cd39l1, and ecto-5’-nucleotidase/Cd73

Donor Age:

Adult

Donor Gender:

N/A

Donor Ethnicity:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm . To make the completed growth medium, add the following components to the base medium: fetal bovine serum to final concentration of 10% and Penicillin/Streptomycin Solution to a final concentration of 1%.
Carbon dioxide (CO₂): 5%, Temperature: 37.0°C.
abm does not recommend to use heat-inactivated FBS for cell culture unless specified otherwise.

Quality Control:

1.) Gene expression analysis to confirm the expression of common liver myofibroblast-associated genes. 2.) Immunoblot and immunofluoresence microscopy to detect myofibroblast-specific cell markers. 3.) Transfection assay to confirm DNA transfectability of cells. 4.) Co-culture assay to demonstrate the inhibition of Mz-ChA-1 cholangiocarcinoma proliferation.

Shipping Condition:

Dry Ice

Storage Condition:

liquid nitrogen or -180C