abm | Pax6 KnockOut U251N Cell Line | T6167
CRISPR-Cas9 was utilized to knockout PAX6 from the U251N cells to establish Pax6 KnockOut U251N Cell Line (2.10 KO cells). Knockout was verified via Western blot and immunocytochemistry. PAX6 KnockOut U251N Cell Line (2.10 KO) may be paired with the rescued Pax6 Stable U251N Cell Line (2.10 R), available at abm
For Research Use Only
The base medium for this cell line is Prigrow III medium available at abm
1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
1) Western blot and immunocytochemistry conducted with rabbit anti-PAX6 antibody (cat#AB2237, Millipore) and mouse anti-Pax6 antibody(Cat# pax6, RRID:AB_528427) to determine knockout.