abm | Pax6 KnockOut U251N Cell Line | T6167

abm | Pax6 KnockOut U251N Cell Line | T6167

CRISPR-Cas9 was utilized to knockout PAX6 from the U251N cells to establish Pax6 KnockOut U251N Cell Line (2.10 KO cells). Knockout was verified via Western blot and immunocytochemistry. PAX6 KnockOut U251N Cell Line (2.10 KO) may be paired with the rescued Pax6 Stable U251N Cell Line (2.10 R), available at abm

Biosafety:

II

Organism:

Human

Source Organ:

Brain

Growth Properties:

Adherent

Morphology:

Triangular cobblestone-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

20 hours

Seeding Density:

N/A

Markers:

N/A

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow III medium available at abm

Preservation:

1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.

Quality Control:

1) Western blot and immunocytochemistry conducted with rabbit anti-PAX6 antibody (cat#AB2237, Millipore) and mouse anti-Pax6 antibody(Cat# pax6, RRID:AB_528427) to determine knockout.

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C