Description
abm | F14-NEDD9 Stable SCC9 Cell Line | T6461
NEDD9, or Neural precursor cell expressed developmentally downregulated 9, has been associated marker for metastatic signatures of melanoma and other cancer such as, breast cancer, glioblastoma, lung cancer and head and neck squamous cell carcinoma. SCC9 cells were transfected with a plasmid carrying F14-NEDD9 with N-terminal EGFP. Stably F14-NEDD9 expressing cells were selected for via Geneticin to establish the F14-NEDD9 Stable SCC9 Cell Line which may be used to further study cancer biology and metastatic properties.
Biosafety:
II
Organism:
Human (H. sapiens)
Source Organ:
Oral
Growth Properties:
Adherent
Morphology:
Epithelial-like
Clones:
N/A
Passage Number:
N/A
Population Doner:
N/A
Seeding Density:
N/A
Markers:
F14-NEDD9, GFP Geneticin-resistance
Applications:
For Research Use Only
Doner Gender:
Male
Donor Ethnicity:
Not disclosed
Knockdown Method:
N/A
Induction:
N/A
Overexpression:
N/A
Freeze Thaw:
N/A
Propagation:
The base medium for this cell line is Prigrow IV medium available at abm
Preservation:
1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
Quality Control:
1) Western blot
Tumorgenicn:
N/A
Shipping Conditions:
Dry Ice
Storage Contidions:
-180°C