abm | GNAO1 R243H Stably Expressing Immortalized Human Mammary Epithelial Cell Line | T6202

abm | GNAO1 R243H Stably Expressing Immortalized Human Mammary Epithelial Cell Line | T6202

GNAO1 R243H Stably Expressing Immortalized Human Mammary Epithelial Cell line expresses GNAO1, the protein of which represent the alpha subunit of the G0 heterotrimeric G-protein signal-transducing complex and is partially responsible for smooth muscle contraction and depressing neuronal activity. This genes role is to inhibit adenylate cyclase , open K+-channels (via β/γ subunits), and close Ca2+-channels. This particular cell line expresses the the Arginine243 → Histidine (R243H) mutation of GNAO1, which has been identified in breast carcinomas and is known to promote oncogenesis when introduced into cells. The oncogenic R234H mutation renders Gαo constitutively active by accelerating the rate of nucleotide exchange, which enhances signaling pathways that are associated with neoplasmic transformation. This cell line was made using retrovirus transduction. It carries flag tag and expresses GFP.

Biosafety:

II

Organism:

Human

Source Organ:

Skin

Growth Properties:

Adherent

Morphology:

Endothelial-like

Clones:

N/A

Passage Number:

N/A

Population Doner:

N/A

Seeding Density:

N/A

Markers:

G418 resistance

Applications:

For Research Use Only

Doner Gender:

N/A

Donor Ethnicity:

N/A

Knockdown Method:

N/A

Induction:

N/A

Overexpression:

N/A

Freeze Thaw:

N/A

Propagation:

The base medium for this cell line is Prigrow IV available at abm

Preservation:

N/A

Quality Control:

N/A

Tumorgenicn:

N/A

Shipping Conditions:

Dry Ice

Storage Contidions:

-180°C