GV3101 ElectroCompetent Agrobacterium

Intact Genomics GV3101 ElectroCompetent Agrobacterium cells are useful for Agrobacterium­-mediated genetic transformation of Arabidopsis thaliana, tobacco, corn, potato, and other plants.

Description

Intact Genomics GV3101 ElectroCompetent Agrobacterium cells are optimized for the highest transformation efficiencies which is ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction.

The GV3101 strain has a C58 chromosomal background with rifampicin resistance and the Ti plasmid pMP90 (pTiC58DT-DNA) with gentamicin resistance. The GV3101 Ti plasmid has the T-DNA region sequences deleted and transformation with a binary vector containing the missing T-region results in a functional T-DNA binary system that allows for transfer of genetic material into a host plant’s genome. Therefore, this system is often used for Agrobacterium-mediated transformation of several dicots such as Arabidopsis thaliana, tobacco, potato, and monocots like corn.

Specifications
Competent cell type: Electrocompetent
Species: A. tumefaciens
Strain: GV3101
Format: Tubes
Transformation efficiency:  ≥ 1 x 10⁷  cfu/µg pCAMBIA1391z  DNA
Blue/white screening: No
Shipping condition: Dry ice

Reagents Needed for One Reaction
GV3101 ElectroComp Agrobacterium: 25 µl
DNA (pCAMBIA1391z, 500 pg/µl):    1 µl
Recovery medium:    1 ml

Storage
GV3101 ElectroComp Agrobacterium: -80 ºC
pCAMBIA1391z control DNA: -20 ºC
Recovery medium:    4 ºC

Quality Control
Transformation efficiency is tested by using the pCAMBIA1391z control DNA supplied with the kit and using the protocol in this manual. Transformation efficiency should be ≥1 x 10⁷ CFU/µg pCAMBIA1391z DNA. Untransformed cells are tested for appropriate antibiotic sensitivity.1282-12 1282-36