PAGE GelRed® Nucleic Acid Gel Stain

PAGE GelRed® is an ultra sensitive, extremely stable, and environmentally safe fluorescent nucleic acid dye specifically designed to stain DNA in polyacrylamide gels.

• Superior dsDNA staining in polyacrylamide gels
• Formulated for efficient penetration of polyacrylamide gel
• Safer than EtBr: non-mutagenic and non-hazardous for disposal
• Simple pre- and post-electrophoresis gel staining, no destaining
• Use with standard EtBr imaging equipment or optical settings
• Compatible with downstream gel purification, restriction digest, sequencing, and cloning

PAGE GelRed™ Nucleic Acid Gel Stain, 10,000X in water

PAGE GelRed™ Nucleic Acid Gel Stain, 1X in water

PAGE GelRed® is a non-toxic, non-mutagenic red DNA gel stain specifically designed to stain DNA in polyacrylamide gels. It can be imaged using a 254 nm UV transilluminator with an ethidium bromide filter and can be removed from DNA gel staining using commonly available gel extraction kits. PAGE GelRed® is 6-8 times more sensitive for dsDNA over RNA. PAGE GelRed® is offered at 10,000X and 1X concentrations. Only the highly concentrated, 10,000X in water, can be used for pre-casting. While both concentrations of PAGE GelRed® can be used as a post-electrophoresis gel stain, 1x PAGE GelRed® is supplied in a 4L Cubitainer® as a convenient and easily dispensed, ready-to-use post-stain.

PAGE GelRed®: A Superior Dye for Polyacrylamide Gels

The original GelRed® and GelGreen® dyes, the dyes’ membrane impermeability was achieved by mainly making the dyes physically large. While this strategy works extremely well to improve the dyes’ safety, the relatively large size of GelRed® and GelGreen® make the dyes difficult to penetrate into the more densely packed polyacrylamide gels, rendering the dyes less optimal for PAGE gel staining.

In designing PAGE GelRed®, we used a novel approach to make the dye membrane impermeable without making the dye large. As a result, PAGE GelRed readily stains dsDNA in densely packed polyacrylamide gels. Importantly, the dye still possesses the benefits that the Original GelRed® is known for; safety, sensitivity, stability, and compatibility with existing instruments and downstream sample analysis. Also see our GelRed® and GelGreen® Frequently Asked Questions (FAQs).

Non-Mutagenic and Safer for the Environment

A series of safety tests have confirmed that PAGE GelRed® is non-cytotoxic, non-mutagenic, and non-hazardous at concentrations well above the working concentrations used in gel staining. As a result, working strength PAGE GelRed® can be safely disposed of down the drain or in regular trash, providing convenience and reducing cost in waste disposal. For detailed test results, you may download the PAGE GelRed® Safety Report.

How Safe is Your Gel Stain?

Many so-called “safe” DNA dyes like SYBR® Safe, Midori Green, GreenSafe, SafeView™, and RedSafe™ not only have low sensitivity, but also readily penetrate living cells to bind DNA, and some are cytotoxic. Unlike these dyes, GelRed® is cell membrane-impermeant, so it cannot enter living cells to interact with their DNA. See our Gel Stains Comparison Flyer or  Gel Stains Comparison White Paper for details.

Choose the Right Stain for Your Application

PAGE GelRed® Nucleic Acid Gel Stain is the recommended stain for staining DNA in polyacrylamide gels. While PAGE GelRed® can be used to stain DNA in agarose gels but will be less sensitive than Original GelRed®. For pre-cast gel staining, only the highly concentrated dye solution, 10,000X in water, can be used by adding the dye directly to molten agarose. While both concentrations of PAGE GelRed® can be used as a post-electrophoresis gel stain, 1X PAGE GelRed® is supplied in a 4L Cubitainer® as a convenient and easily dispensed, ready-to-use post-stain.

For agarose gels, we recommend using Original GelRed® Nucleic Acid Gel Stain or GelGreen® Nucleic Acid Gel Stain. While PAGE GelRed® can be used to stain DNA in agarose gels, it will be less sensitive than the Original GelRed®. GelRed® 3X in water is ready-to-use for post-electrophoresis gel staining, and is supplied in a 4L Cubitainer®. Higher concentrations of Original GelRed® are available as 10,000X in water or DMSO. GelRed® in water is a newer, safer formulation and our recommended format. We continue to offer GelRed® in DMSO for established users who do not wish to alter their protocols. We also offer GelRed® Agarose and GelRed® Prestain Plus 6X Loading Dye. GelGreen® Nucleic Acid Gel Stain, a safer replacement for SYBR® gel stains, which is compatible with visible light excitation. For more information, view our DNA Gel Stains Technology Page.