BLOOD AGAR BASE | DM100

Blood Agar Base (B.A.B.) | DM100
A general purpose medium that gives typical haemolytic reactions when the medium is supplemented with sterile defibrinated blood.

Mast Blood Agar Base can be used without enrichment for the isolation and cultivation of many organisms. Enriched with blood, it gives good growth of fastidious organisms and clearly demonstrates typical haemolytic activity. It may be used in the preparation of heated blood ("chocolate") agar, which is especially suitable for the cultivation of Haemophilus spp. and Neisseria spp. It has been shown to be superior to several other blood agar bases available

2. Technical Formula*

3. Directions
1. Suspend by swirling 37.5g of powder in 1 litre or the contents of the sachet in the stated volume of distilled or deionised water.
2. Autoclave at 121°C (15p.s.i.) for 15 minutes.
3. Thoroughly mix before pouring

4. In Use
"Blood Agar"
Cool the base to about 50°C and add aseptically 5-7% sterile defibrinated blood. Agitate gently to disperse the blood and
pour.

"Chocolate Agar"
Add 7% defibrinated blood to the base at 75°C and maintain at this temperature for 10 minutes. Agitate gently and pour. Alternatively, heated blood plates may be prepared by holding poured plates at 55°C for 1-2 hours.