Ampdirect Plus

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Ampdirect Plus | 241-08800-98 from Nacalai Tesque is available for delivery.

Ampdirect Plus is a novel PCR buffer that effectively neutralizes DNA polymerase inhibitors present in tissues or body fluids from plants or animals, and allows direct PCR amplification from as little as 0.5 µl of sample.

* Ampdirect Plus is produced by SHIMADZU BIOTECH in JAPAN.


  • Direct PCR amplification from tissues or body fluids (including blood) from plants or animals
  • No DNA extraction required
  • Minimizes the risk of sample cross contamination
  • Requires small volume of blood (0.5 - 1 µl)
  • Compatible with various anticoagulants (including sodium citrate, dipotassium EDTA and sodium heparinate)
  • Compatible with blood stored frozen for long periods
  • Ideal for genotyping and genetic screening experiments

Application 1

Direct PCR of 5 Genes from 23 Different Human Blood Samples

  • PCR: 23 different human blood samples (1L sample volumes treated with EDTA was added to 50 l of Ampdirect Plus mixture)
  • Lane M: Molecular size marker


Application 2

Direct PCR from fresh and frozen blood

  • Target: Human beta-globin gene
  • PCR: 5 l (lane 1), 2.5 l (lane 2), 1.25 l (lane 3), 0.63 l (lane 4) and 0 l (lane N) of human blood / 50 l of Ampdirect mixture
  • Molecular size marker: ΦX174 RF DNA digested with HincII (lane M)

Ordering Information

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1 Review

  • 4


    Posted by Micah on 26th Sep 2022

    like their name Gentaur, their products are totally organic. No use of any harmful chemicals is really happy with the products.

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