Recombinant Core Streptavidin (r-cSA)
- SKU:
- GEN-NRPA09S
- Formulation:
- lyophilized from 5 mM of PB (4 mM of Na2HPO4, 1 mM of NaH2PO4, pH 7.4)
- Mol. Weight:
- 13.5 kDa subunit; Tetramer 54kDa
- Theory pI:
- 6.04
- Theoretical activity:
- 18.1 U / mg protein (rc-SA: Biotin = 1: 4 (mol: mol))
- Resources:
- Escherichia coli (E. coli)
- Purity:
- ≥95% by SDS-PAGE analysis
- Test activity:
- ≥ 15 U / mg protein (determined by NUPTEC according to the modified green method)
- Storage condition:
- -20 °C
- Storage period:
- 3 years
Description
Streptavidin is a homotetrameric protein found in the culture broth of Streptomyces avidinii. Similar to avidin, one mole of streptavidin can bind to 4 moles of biotin with high affinity virtually unmatched in nature. Streptavidin lacks the carbohydrate side chains present on avidin and has an isoelectric point close to neutrality. Therefore, it has a reducing nonspecific binding level compared to avidin. Streptavidin has been widely applied in various biological fields, such as ELISA, IHC, TRFIA, PCR quantification, single-stranded nucleotide isolation, biomolecule purification, and monoclonal antibody production.
Compared to native streptavidin, rc-SA is improved in stability and solubility by removing the sequence unrelated to activity. In addition, a cysteine residue is inserted at the C-terminus of rc-SA for covalent conjugation to the resin.
M ： Protein molecular weight marker
Channel 1 ： rc-SA subunit
Lane 2 ： rc-SA homotetramer
For research only!
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