Mouse Anti S-AdenosylHomocysteine (SAH) Clone 844-2 | MA00306

Mouse Anti S-AdenosylHomocysteine (SAH) Clone 844-2 | MA00306 | Arthus Biosystems

Product name        

Mouse anti-SAH 6a/b

Catalog Number    

MA00306-50/100

Description              

Mouse monoclonal antibody against S-Adenosylhomocysteine [844-2]

Specificity                 

MA00306 shows the following reactivities with related compounds: S-Adenosylhomocysteine: 100%, S-Adenosylmethionine: -2.1°A, Adenosine: < 1%, Homocysteine: < 1%, L-Cysteine: < 1%, Glutathione:< 1%, L-Cystathionine: < 1%, Methythioadenosine (MTA): < 5%, ADP (adenosine diphosphate): < 1%, ATP (adenosine triphosphate): < 1%

Immunogen              

S-Adenosylhomocysteine conjugated to BSA

Properties

Form                                   

Liquid

Storage instructions          

Store at 4°C, -20°C for long term storage

Storage buffer                    

PBS 10mM pH7.4 (NaCI 150mM), Sodium azide 0.02%, BSA 10mg/m1 or PBS 10mM, pH7.4 (NaCI 150mM), Sodium azide 0.02%, Glycerol 50%, BSA 10mg/m1

Purity                                 

>95% Purified from mouse ascites fluid by affinity chromatography

Clonality                             

Monoclonal

Clone number                    

844-2

Immunoglobin isotype      

IgG2a

Affinity                               

Ka = 2.34 x 109L/mol ( 4.27 x 10-19M )

Research Areas                  

• Methylation of biomolecules (DNA, RNA, proteins, hormones, neurotransmitters, etc.)
• One-carbon metabolism
• Signal Transduction
• Metabolism
• Pathways and Processes Cancers
• Arthritis
• Heart diseases
• Neurodegenerative diseases Atherosclerosis
• Liver diseases
• Kidney diseases

Applications

The use of MA00306 in the following tested applications has been tested. The titer was measured using indirect assay with the concentration of the antibody at 1mg/ml. The application notes include recommended starting dilutions. Optimal
dilutions/concentrations should be determined by the end user. Higher dilution than suggested maybe used in IHC and IF. The product may be used in other not-yet-tested applications.

Notes

• cELISA:  1:8,000/20,000
• FCM:  1:200
• IHC:  1:200

Target

S-adenosylhomocysteine is a competitive inhibitor of S-adenosylmethionine-dependant methyl transferase reactions. Therefore, it plays a key role in the control of methylation via regulation of the intracellular concentration of S-adenosylhomocysteine.

Cellular localization  

Cytoplasm, nuclear

Figure 1:  Titration curve for purified monoclonal antibody 844-2 at 0.45mg/ml.

Figure 2: Competitive ELISA using anti-S-Adenosymethionine monoclonal antibody [844-2] (MA00306)

The 0.2 pg/ml of SAH-BSA was coated into 96 wells. Serial dilution of SAH standard (SAHNa), S-Adenosylmethionine ( SAM: Sigma-Aldrich Cat# A2408),  Homocysteine (H-Cys),  L-Cysteine (L-Cys), Adenosine (Ade),  Glutathione (GST),  L-Cystathionine (L-CTT), Methythioadenosine (MTA), ADP (adenosine diphosphate), ATP (adenosine triphosphate) and  properly diluted  MA00306 were added. HRP conjugated Goat anti-Mouse IgG antibody was used to develop the color. OD450 value was measured on each well.

Figure 3: Immunohistochemistry staining was performed using MA00306 with benign liver tissue adjacent to liver cancer. Brown areas indicated strong positive staining in nuclear and cytoplasmic areas (x400).