HRP Anti-SAM Monoclonal Antibody Clone 118-18 | MAH00203-50

HRP Anti-SAM Monoclonal Antibody Clone 118-18 | MAH00203-50 | Arthus Biosystems

Product name         

HRP-anti-SAM 3a

Catalog Number     

MAH00203-50

Description              

Horseradish peroxidase (HRP) conjugated anti-S-adenosylmethionine monoclonal antibody clone 118-18

Specificity                

MAH00203 shows the following reactivities with related compounds: S-Adenosylmethionine: 100%, S-Adenosylhomocysteine: < 1% ,Adenosine: < 1%, L-Methionine: < 1%, Methythioadenosine (MTA): < 1%, ADP (adenosine diphosphate): < 1%, ATP (adenosine triphosphate): < 1%

Immunogen            

S- Adenosylmethionine analog Aza-SAM conjugated to KLH.

Properties

Form                                   

Liquid

Storage instructions          

Store at 4°C, -20°C for long term storage

Storage buffer                    

PBS 10mM pH 7.4 (NaCI 150mM), Proclin 0.1%, Glycerol 50%, BSA 10mg/mi

Purity                                 

>95% Purified from mouse ascites fluid by affinity chromatography

Clonality                             

Monoclonal

Clone number                    

118-18

Immunoglobin isotype      

IgG2b

Research Areas                  

• Methylation of biomolecules (DNA, RNA, proteins, hormones, neurotransmitters, etc.)
• One-carbon metabolism
• Signal Transduction
• Metabolism
• Pathways and Processes Cancers
• Arthritis
• Heart diseases
• Neurodegenerative diseases
• Atherosclerosis
• Liver diseases
• Kidney diseases

Applications

The use of MAH00203 in the following tested applications has been tested. The application notes include recommended starting dilutions. Optimal dilutions/concentrations should be determined by the end user. Higher dilution than suggested maybe used in IHC and IF. The product may be used in other not-yet-tested applications. Refer to Cat # MA00203 for details.

Notes

cELISA: 1/2,000 - 1/10,000 (depending on the amount of coating)

Figure 1: The standard curve with HRP-anti-SAM 3 in direct competitive ELISA using 4-parameter logistic non-linear regression model. The 0.25 ug/m1 of SAM coating standard (Cat # ACT00201) was coated on micro-titer wells. Serial dilution of SAM standard (Cat # AST00201) was added along with 1:10,000 diluted HRP-anti-SAM 3 and was incubated for about 60 minutes. The substrates were then added and OD450 was measured.