SARS-CoV-2 Lineage B.1.617.2 Delta Variant (USA/PHC658/2021) CF (Heat Inactivated)

SARS-CoV-2 Lineage B.1.617.2 Delta Variant (Isolate: USA/PHC658/2021) is an enveloped, posititve-sense single-standed RNA virus from th Coronaviridae family and the Betacoronaviridae genus. Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), isolate hCoV-19/USA/PHC658/2021 was isolated in Memphis. Tenessee, USA in May 2021. Under the nomenclature system introduced by GISAID (Global Initiative on sharing All influenza Data), SARS-CoV-2, isolate hCoV-19/USA/PHC658/2021 is assigned lineage B.1.617.2 using Phylogenetic Assignment of named Global Outbreak LINeage (PANGOLIN) tool. SARS-CoV-2, lineage B.1.617.2 was first detected in India and labelled as a variant of concern (VOC). It was labelled as  Delta variant by the World Health Organization (WHO).

Each frozen aliquot contains 1mL of heat inactivated viral culture fluid by centrifugation to remove cellular debris. The pre-inactivation titer was determined from an infectious aliquot.

Viral inactivation is verified after heat inactivation by the absence of viral growth in tissue culture-based infectivity assays.

INTENDED USE:

Heat inactivated viral culture fluids are sold as consumable testing materials. The suitability and performance characteristics should be determined by your laboratory for each intended usage.

These products ar NOT intended for use in the manufacture or processing of injectable products subject to licensure under section 351 of the Public Health Service Act or for any other product intended for administration to humans.

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

BIOSAFETY:

Inactivated SARS-COV-2 should be used within a Biological Safety Level 2 facility or biosafety cabinet. Please consult your institution's regulations regarding the use of this product. For a detailed discussion on biological safety see the 5th edition of Biosafety in Microbiological and Biomedical laboratories (BMBL), published by the CDC at http:www.cdc.gov/biosafety/publications/bmbl5/index.htm

PRECAUSIONS:

 - Use Universal Precautions, this product is potentially biohazardous.

 - Repetitive freezing and thawing is not recommended (aliquot materials if necessary).

 - To avoid cross-contamination, use separate pipette tips for all reagents.

RECOMMENDED STORAGE:

Heat inactivated viral culture fluids should be stored at -65°C or below.

*The following reagent was obtained through BEI Resources, NIAD, NIH: SARS-Related Coronavirus 2, Isolate hCoV-19/USA/CA-Stanford-15_S02/2021 (Lineage B.1.617.1; Kappa Variant), BR-55486, contributed by Dr. Mehul Suthar and Dr. Benjamin Pinsky.