Specific Enrichment - Multiple Applications
Kinase (& ATP binding proteins) enrichment reagent
- Reversible immobilization of phosphate group with optimal nucleotide orientation & specificity
- Enrichment 3-5X, binding protein recoverable, ~200 μg
- 60 minute, scaleable protocol compatible with functional assays, electrophoresis and LC-MS
- Phosphatase activity & cyclic nucleotide phosphodiesterase (PDE) activity not detectable
- Improves protein normalization when comparing heterogeneous tissues
- On-bead digestion protocols for LC-MS IDs
Glycoprotein Enrichment Using Phenyl Boronic Acid
- Enriches heterogeneous sets of glycoprotein’s, N-linked & O-linked
- Consumable, no column regeneration
- Species and tissue agnostic
- Sorbitol elution; compatible with functional assays, electrophoresis and LC-MS
- Binds biomolecules containing 1,2 cis-diol groups
- Chemically derived, ideal for glyco-proteomic applications
- NuGel™ polymer coating, porous silica based
- Supplied as bead only (dry powder) or as kit (includes all binding and elution buffers)
| Sample Type | %Glycoprotein (Sorbitol Elution) |
|---|
| Mouse Plasma |
33 |
| Rat Serum |
44 |
| Sheep Serum |
18 |
| Bovine Serum |
40 |
| Bovine Brain Homogenate |
9 |
Virus Enrichment & Purification
- Purifies whole infectious non-enveloped virus, isolates antigenic virions
- Enriches for viral proteins and nucleic acids
- Use with (proven 350X enrichment) or without ultracentrifugation
- Used for monitoring vaccine immune response
Aqueous Protein Crash & Enrichment of Metabolites & Analytes
- Serum and plasma protein removal, >95%
- Aqueous protein crash
- < 30 minute protocol
- Applicable for target analytes, drug binding/screening and metabolomics
- Supplied as suspension reagent or NuGel™ dry powder format
