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RNase A Solution | TBS6024

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TBS6024
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Description

RNase A Solution | TBS6024

RNase A (Ribonuclease A) is a single chain polypeptide with 4 disulfied bridges. It is an endoribonuclease that specifically cleaves single stranded RNA at C and U residues. It cleaves the phosphodiester bond between the 5’ribose of a nucleotide and the phosphate group attached to the 3′-ribose of an adjacent pyrimidine nucleotide. The resulting 2′, 3′-cyclic phosphate is hydrlized to the corresponding 3′-nucleoside phosphate.

Applications:

  • Plasmid and genomic DNA preparation.
  • Removal of RNA from recombinant protein preparations.
  • Ribonuclease protection assays. Can also be used in conjunction with RNase T1.
  • Mapping single-base mutations in DNA or RNA

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