AffiVET® Dog Rabies Antigen Rapid Test Card

Dog Rabies virusantigen rapid test card  

1. Product Usage

Rabies is a natural epidemic acute infectious disease that can be seen in humans and animals. It can be found in canines and felines.Domestic dogs can also become asymptomatic carriers.

This product uses rapid immunochromatography to detect rabies virus antigen in canine saliva secretions.

2. Principle

This product uses rapid immunochromatography to detect Rabies antigen. After the diluted sample is added to the “S” hole on test card, if the Rabies antigen is present in the sample, the antigen will specifically bind to the colloidal gold-labeled Rabies monoclonal antibody, and the complex will move along the chromatographic membrane and be chromatographed. The pre-coated Rabies monoclonal antibody on the membrane is captured, and a wine red detection line is formed at the T-position of the cartridge. If there is no Rabies antigen in the sample, no visible lines will be formed at the T line. In addition, the system has also designed a C-line for verifying that the test is valid—whether negative or positive, the line should be colored, otherwise it will be invalid.

3. Components

1)Rabies Ag Test Card ( Including 1 Rabies Ag test cassette, 1 dropper, 1 desiccant)  10 pieces

2) Sample buffer (1ml/tube) => 10 tubes

3) Swab => 10 pieces

4) Instruction => 1 piece

4. Test Procedure

1) Read the manual carefully and return the test card, sample into room temperature 15~25℃before use.

2) Sample collection: Saliva was collected with a cotton swab.

Immediately insert the cotton swab into the tube containing the sample buffer, repeatedly rotate the cotton swab on the tube wall at least 10 times, and mix it evenly to dissolve the sample as much as possible in the buffer. Squeeze the cotton swab on the wall of the tube above the liquid level to allow the liquid to be squeezed out as much as possible and discard the cotton swab.

3) Open the package, take out test card, put it on desk flatly.

4) Use the supplied dropper to absorb the up-layer clear bright liquid, drop 3~4 drops (about 100ul) into “S” hole on test card.

5) Wait for 10min and read the results, result is invalid beyond 15 min.

5.  Result judgment

1) Positive(+): Both on T Line and C line being seen wine-red color reaction, it means there is Rabies antigen in the sample.

2) Negative(-): no color reaction on Test Line (T Line),only on Control Line(C Line) being seen wine-red color reaction, it means there is no Rabies antigen in the sample.

3) Invalid(X): No color reaction on C Line, it means the operation is wrong or the test card is invalid, test again.

6. Specifications, Storage and Stability

Package: 10T/box

Store at 4~30℃ in a cool, dark and dry place,no frozen. Shelf life: 18 months.

7. Precautions

1) Please use the test card within the shelf life and within 1 hour after opening.

2) Avoid direct sunlight and direct fan blowing during testing.

3) Try not to touch the white film surface of the non-plastic part of the test card.

4) Sample droppers should not be mixed to avoid cross contamination.

5) Do not use sample buffer that are not supplied with this reagent.

6) The used test card should be treated as microbiological dangerous goods.

8. Application limitations

This product is an immunological diagnostic kit, which is only used to provide qualitative test results for clinical testing of pet diseases. If there is any doubt about the test results, please use other methods of diagnosis (such as RT-PCR, Western-blot, etc.) to further analyze the samples. For pathological analysis, please consult your local pet physician.