PCR

PCR

PCR Animation

If the nucleotide sequences at the ends of a particular DNA region are known, the intervening fragment can be amplified directly by the polymerase chain reaction (PCR). Here we describe the basic PCR technique and three situations in which it is used.

The PCR depends on the ability to alternately denature (melt) double-stranded DNA molecules and renature (anneal) complementary single strands in a controlled fashion. As in the membrane-hybridization assay described earlier, the presence of noncomplementary strands in a mixture has little effect on the base pairing of complementary single DNA strands or complementary regions of strands. The second requirement for PCR is the ability to synthesize oligonucleotides at least 18–20 nucleotides long with a defined sequence. Such synthetic nucleotides can be readily produced with automated instruments based on the standard reaction scheme.

A typical PCR procedure begins by heat-denaturation of a DNA sample into single strands. Next, two synthetic oligonucleotides complementary to the 3' ends of the target DNA segment of interest are added in great excess to the denatured DNA, and the temperature is lowered to 50–60 C. These specific oligonucleotides, which are at a very high concentration, will hybridize with their complementary sequences in the DNA sample, whereas the long strands of the sample DNA remain apart because of their low concentration. The hybridized oligonucleotides then serve as primers for DNA chain synthesis in the presence of deoxynucleotides (dNTPs) and a temperature-resistant DNA polymerase such as that from Thermus aquaticus (a bacterium that lives in hot springs).

This enzyme, called Taq polymerase, can remain active even after being heated to 95 C and can extend the primers at temperatures up to 72 C. When synthesis is complete, the whole mixture is then heated to 95 C to melt the newly formed DNA duplexes. After the temperature is lowered again, another cycle of synthesis takes place because excess primer is still present. Repeated cycles of melting (heating) and synthesis (cooling) quickly amplify the sequence of interest. At each cycle, the number of copies of the sequence between the primer sites is doubled; therefore, the desired sequence increases exponentially—about a million-fold after 20 cycles—whereas all other sequences in the original DNA sample remain unamplified.

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  • HAV RT-PCR Qual

    HAV RT-PCR Qual (CE) | V4-50FRT

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  • HPV genotypes 21 RT-PCR Quant NEW

    HPV genotypes 21 RT-PCR Quant NEW (CE) | V21-100FRT

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    HPV genotypes 21 RT-PCR Quant NEW | V21-100FRT from Sacace Biotechnologies is available for delivery Description: General information: Real Time PCR test for quantitative detection and genotyping of HPV (6, 11, 16,18, 26, 31, 33, 35, 39, 44, 45, 51,...
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  • HPV 6/11 RT-PCR

    HPV 6/11 RT-PCR (CE) | V11-100FRT

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  • HPV High Risk Typing

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  • HPV 16/18 RT-PCR Quant

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  • HPV genotypes 14 RT-PCR Quant

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  • HPV High Risk Screen (12 types)

    HPV High Risk Screen (12 types) (CE) | V-26-100F

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  • HPV 14 Screen & 16,18,45 Typing RT-PCR Quant

    HPV 14 Screen & 16,18,45 Typing RT-PCR Quant (CE) | V31-100/F FRT

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  • Cryptococcus neoformans RT-PCR

    Cryptococcus neoformans RT-PCR | F4-100FRT

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  • Pneumocystis jirovecii (carinii) RT-PCR

    Pneumocystis jirovecii (carinii) RT-PCR (CE) | P2-50FRT

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  • HLA B*5701 RT-PCR

    HLA B*5701 RT-PCR (CE) | H53-100FRT

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  • HIV DNA RT-PCR Qual

    HIV DNA RT-PCR Qual | R-V1-D

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  • HIV RT-PCR Quant Dx

    HIV RT-PCR Quant Dx | V0-96/3FRT

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  • ProstateScreen RT-PCR

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  • Gardnerella vaginalis RT-PCR

    Gardnerella vaginalis RT-PCR (CE) | B7-100FRT

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  • Treponema pallidum RT-PCR

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  • Candida albicans RT-PCR

    Candida albicans RT-PCR (CE) | F1-100FRT

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  • T. vaginalis/N.gonorrhoeae/Chl.trachomatis RT-PCR

    T. vaginalis/N.gonorrhoeae/Chl.trachomatis RT-PCR (CE) | B83-100FRT

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  • Chl. trachomatis/N.gonorrhoeae/M.genitalium RT-PCR

    Chl. trachomatis/N.gonorrhoeae/M.genitalium RT-PCR (CE) | B67-100FRT

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  • T. vaginalis/N.gonorrhoeae RT-PCR

    T. vaginalis/N.gonorrhoeae RT-PCR (CE) | B65-100FRT

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  • N.gonor./?.trachomatis/T.vaginalis/M.genitalium RT-PCR

    N.gonor./?.trachomatis/T.vaginalis/M.genitalium RT-PCR (CE) | B61-100FRT

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  • C.trachomatis/Ureapl./M.hominis/M.genitalium RT-PCR

    C.trachomatis/Ureapl./M.hominis/M.genitalium RT-PCR (CE) | B60-100FRT

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  • Chlamydia trachomatis/Ureaplasma/M.genitalium RT-PCR

    Chlamydia trachomatis/Ureaplasma/M.genitalium RT-PCR (CE) | B46-100FRT

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  • Chlamydia trachomatis/Ureaplasma/M.hominis RT-PCR

    Chlamydia trachomatis/Ureaplasma/M.hominis RT-PCR (CE) | B43-100FRT

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  • Trichomonas vaginalis RT-PCR

    Trichomonas vaginalis RT-PCR (CE) | B6-100FRT

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  • Mycoplasma genitalium RT-PCR

    Mycoplasma genitalium RT-PCR (CE) | B4-100FRT

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  • Mycoplasma hominis RT-PCR

    Mycoplasma hominis RT-PCR (CE) | B3-100FRT

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  • Ureaplasma parvum/urealyticum Quant RT-PCR

    Ureaplasma parvum/urealyticum Quant RT-PCR (CE) | B19-100FRT Q

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  • Ureaplasma parvum/urealyticum RT-PCR

    Ureaplasma parvum/urealyticum RT-PCR (CE) | B19-100FRT

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  • Ureaplasma species RT-PCR

    Ureaplasma species RT-PCR (CE) | B2-100FRT

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  • Neisseria gonorrhoeae RT-PCR

    Neisseria gonorrhoeae RT-PCR (CE) | B5-100FRT

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  • Chlamydia trachomatis A,B,C Typing RT-PCR

    Chlamydia trachomatis A,B,C Typing RT-PCR | B86-100FRT

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    Chlamydia trachomatis A,B,C Typing RT-PCR | B86-100FRT from Sacace Biotechnologies is available for delivery Description: General information: Real Time PCR kit for differenziation of A, B, C Types of C.trachomatis Target Disease Type: Sexually...
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  • Chlamydia trachomatis RT-PCR

    Chlamydia trachomatis RT-PCR (CE) | B1-100FRT

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  • LIPC (-250 G>A) SNP-Screen

    LIPC (-250 G>A) SNP-Screen | T01161

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    LIPC (-250 G>A) SNP-Screen | T01161 from Sacace Biotechnologies is available for delivery Description: RT PCR test for detection of Hepatic lipase gene mutation (rs2070895) for Lipid Metabolism Available Formats: - Ready to Use 0,2 mL Tube- Ready to...
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  • PON1 (Gln192Arg) SNP-Screen

    PON1 (Gln192Arg) SNP-Screen | T01125

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    PON1 (Gln192Arg) SNP-Screen | T01125 from Sacace Biotechnologies is available for delivery Description: RT PCR test for detection of paraoxonase 1 gene mutation (575A>G, rs662) for Lipid Metabolism Available Formats: - Ready to Use 0,2 mL Tube- Ready...
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  • LPL (Ser447Ter) SNP-Screen

    LPL (Ser447Ter) SNP-Screen | T01149

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    LPL (Ser447Ter) SNP-Screen | T01149 from Sacace Biotechnologies is available for delivery Description: RT PCR test for detection of lipoprotein lipase gene mutation (1421C>G, rs328) for Lipid Metabolism Available Formats: - Ready to Use 0,2 mL...
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  • IL17A (G-197A) SNP-Screen

    IL17A (G-197A) SNP-Screen | T01171

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    IL17A (G-197A) SNP-Screen | T01171 from Sacace Biotechnologies is available for delivery Description: RT PCR test for detection of Interleukin-17A gene mutation (rs2275913) for Immunity Available Formats: - Ready to Use 0,2 mL Tube - Ready to Use 12 x...
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  • TNF (G-308A) SNP-Screen

    TNF (G-308A) SNP-Screen | T01177

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    TNF (G-308A) SNP-Screen | T01177 from Sacace Biotechnologies is available for delivery Description: RT PCR test for detection of Tumor necrosis factor mutation (rs1800629) for Immunity Available Formats: - Ready to Use 0,2 mL Tube - Ready to Use 12 x 8...
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  • APOE Leu48Pro SNP-Screen

    APOE Leu48Pro SNP-Screen | T01148

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    APOE Leu48Pro SNP-Screen | T01148 from Sacace Biotechnologies is available for delivery Description: RT-PCR test for detection of apolipoprotein E mutation (T3100C, rs769452) for Myocardial infarction, ischemic stroke Available Formats: - Ready to Use...
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  • ACE Alu Ins/Del SNP-Screen

    ACE Alu Ins/Del SNP-Screen | T01272

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    ACE Alu Ins/Del SNP-Screen | T01272 from Sacace Biotechnologies is available for delivery Description: RT-PCR test for detection of angiotensin II receptor, type 1 mutation (rs5186) for Hypertension Available Formats: - Ready to Use 0,2 mL Tube - Ready...
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  • AGTR1 (A1166C) SNP-Screen

    AGTR1 (A1166C) SNP-Screen | T01131

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    AGTR1 (A1166C) SNP-Screen | T01131 from Sacace Biotechnologies is available for delivery Description: RT-PCR test for detection of angiotensin II receptor, type 1 mutation (rs5186) for Hypertension Available Formats: - Ready to Use 0,2 mL Tube - Ready...
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  • NOS3 (C786T) SNP-Screen

    NOS3 (C786T) SNP-Screen | T01182

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    NOS3 (C786T) SNP-Screen | T01182 from Sacace Biotechnologies is available for delivery Description: RT-PCR test for detection of Nitric oxide synthase 3 mutation (rs2070744) for Hypertension Available Formats: - Ready to Use 0,2 mL Tube - Ready to Use...
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  • AGT 2 (C4072T) SNP-Screen

    AGT 2 (C4072T) SNP-Screen | T01119

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    AGT 2 (C4072T) SNP-Screen | T01119 from Sacace Biotechnologies is available for delivery Description: RT-PCR test for detection of Angiotensinogen mutation 2 (Met235Thr, rs699) for Hypertension Available Formats: - Ready to Use 0,2 mL Tube - Ready to...
    441-T01119
    Select Currency //742.00 - Select Currency //1,002.00
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  • AGT 1 (C521T) SNP-Screen

    AGT 1 (C521T) SNP-Screen | T01118

    Select Currency //742.00 - Select Currency //1,002.00
    AGT 1 (C521T) SNP-Screen | T01118 from Sacace Biotechnologies is available for delivery Description: RT-PCR test for detection of Angiotensinogen mutation 1 (Thr207Met, rs4762) for Hypertension Available Formats: - Ready to Use 0,2 mL Tube - Ready to...
    441-T01118
    Select Currency //742.00 - Select Currency //1,002.00
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  • IL28B (C>T) SNP-Screen

    IL28B (C>T) SNP-Screen | T01371

    Select Currency //742.00 - Select Currency //1,002.00
    IL28B (C>T) SNP-Screen | T01371 from Sacace Biotechnologies is available for delivery Description: RT-PCR test for detection of Interleukin mutation 2 (rs12979860) for Hepatitis C treatment prognosis Available Formats: - Ready to Use 0,2 mL Tube-...
    441-T01371
    Select Currency //742.00 - Select Currency //1,002.00
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  • IL28B (T>G) SNP-Screen

    IL28B (T>G) SNP-Screen | T01349

    Select Currency //742.00 - Select Currency //1,002.00
    IL28B (T>G) SNP-Screen | T01349 from Sacace Biotechnologies is available for delivery Description: RT-PCR test for detection of Interleukin mutation 1 (rs8099917) for Hepatitis C treatment prognosis Available Formats: - Ready to Use 0,2 mL Tube -...
    441-T01349
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  • FTO (A23525T) SNP-Screen

    FTO (A23525T) SNP-Screen | T01329

    Select Currency //742.00 - Select Currency //1,002.00
    FTO (A23525T) SNP-Screen | T01329 from Sacace Biotechnologies is available for delivery Description: RT-PCR test for detection of FTO gene mutation (rs9939609) for Diabetes and obesity Available Formats: - Ready to Use 0,2 mL Tube - Ready to Use 12 x 8...
    441-T01329
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  • SELPLG (G186A) SNP-Screen

    SELPLG (G186A) SNP-Screen | T01357

    Select Currency //742.00 - Select Currency //1,002.00
    SELPLG (G186A) SNP-Screen | T01357 from Sacace Biotechnologies is available for delivery Description: RT-PCR test for detection selectin P ligand gene mutation (Met62Ile; rs2228315) for Coagulation/Fibrinolysis System Available Formats: - Ready to Use...
    441-T01357
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  • GPIBA1b (T -5C ) SNP-Screen

    GPIBA1b (T -5C ) SNP-Screen | T01354

    Select Currency //742.00 - Select Currency //1,002.00
    GPIBA1b (T -5C ) SNP-Screen | T01354 from Sacace Biotechnologies is available for delivery Description: RT-PCR test for detection of platelet glycoprotein Ib? gene mutation (Kozak sequence polymorphism; rs2243093) for Coagulation/Fibrinolysis System ...
    441-T01354
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  • GPIBA1b (C482T) SNP-Screen

    GPIBA1b (C482T) SNP-Screen | T01179

    Select Currency //742.00 - Select Currency //1,002.00
    GPIBA1b (C482T) SNP-Screen | T01179 from Sacace Biotechnologies is available for delivery Description: RT-PCR test for detection of platelet glycoprotein Ib? gene mutation (Thr145 Met;rs6065) for Coagulation/Fibrinolysis System Available Formats: -...
    441-T01179
    Select Currency //742.00 - Select Currency //1,002.00
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  • ITGB3 (T176C) SNP-Screen

    ITGB3 (T176C) SNP-Screen | T01106

    Select Currency //742.00 - Select Currency //1,002.00
    ITGB3 (T176C) SNP-Screen | T01106 from Sacace Biotechnologies is available for delivery Description: RT-PCR test for detection of Integrin beta-3 gene mutation (Leu33Pro; rs5918) for Coagulation/Fibrinolysis System Available Formats: - Ready to Use 0,2...
    441-T01106
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  • ITGA2 (C807T) SNP-Screen

    ITGA2 (C807T) SNP-Screen | T01155

    Select Currency //742.00 - Select Currency //1,002.00
    ITGA2 (C807T) SNP-Screen | T01155 from Sacace Biotechnologies is available for delivery Description: RT-PCR test for detection of Integrin alpha2 gene mutation (Phe224Phe;rs1126643) for Coagulation/Fibrinolysis System Available Formats: - Ready to Use...
    441-T01155
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  • FXIII (G103T) SNP-Screen

    FXIII (G103T) SNP-Screen | T01355

    Select Currency //742.00 - Select Currency //1,002.00
    FXIII (G103T) SNP-Screen | T01355 from Sacace Biotechnologies is available for delivery Description: RT-PCR test for detection of factor XIII A1 gene mutation (Val35Leu; rs5985) for Coagulation/Fibrinolysis System Available Formats: - Ready to Use 0,2...
    441-T01355
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  • FXII (C -4T) SNP-Screen

    FXII (C -4T) SNP-Screen | T01356

    Select Currency //742.00 - Select Currency //1,002.00
    FXII (C -4T) SNP-Screen | T01356 from Sacace Biotechnologies is available for delivery Description: RT-PCR test for detection of factor XII gene mutation (rs1801020) for Coagulation/Fibrinolysis System Available Formats: - Ready to Use 0,2 mL Tube -...
    441-T01356
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  • FGB (G-455A) SNP-Screen

    FGB (G-455A) SNP-Screen | T01107

    Select Currency //742.00 - Select Currency //1,002.00
    FGB (G-455A) SNP-Screen | T01107 from Sacace Biotechnologies is available for delivery Description: RT-PCR test for detection of fibrinogen beta gene (rs1800790) for Coagulation/Fibrinolysis System Available Formats: - Ready to Use 0,2 mL Tube - Ready...
    441-T01107
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  • PAI SERPINE (675 5G/4G) SNP-Screen

    PAI SERPINE (675 5G/4G) SNP-Screen | T01120

    Select Currency //742.00 - Select Currency //1,002.00
    PAI SERPINE (675 5G/4G) SNP-Screen | T01120 from Sacace Biotechnologies is available for delivery Description: PCR test for detection of polymorphism of SERPINE1/PAI1 gene (rs1799768) for Coagulation/Fibrinolysis System Available Formats: - Ready to...
    441-T01120
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  • MTR (A2756G) SNP-Screen

    MTR (A2756G) SNP-Screen | T01143

    Select Currency //742.00 - Select Currency //1,002.00
    MTR (A2756G) SNP-Screen | T01143 from Sacace Biotechnologies is available for delivery Description: RT-PCR test for detection of methionine synthase gene mutation (rs1805087) for Coagulation/Fibrinolysis System Available Formats: - Ready to Use 0,2 mL...
    441-T01143
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  • MTRR (A 66 G) SNP-Screen

    MTRR (A 66 G) SNP-Screen | T01124

    Select Currency //742.00 - Select Currency //1,002.00
    MTRR (A 66 G) SNP-Screen | T01124 from Sacace Biotechnologies is available for delivery Description: RT-PCR test for detection of methioninesynthase gene mutation (Ile22Met; rs1801394) for Coagulation/Fibrinolysis System Available Formats: - Ready to...
    441-T01124
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  • MTHFR (C677T) SNP-Screen

    MTHFR (C677T) SNP-Screen (CE) | T01103

    Select Currency //742.00 - Select Currency //1,002.00
    MTHFR (C677T) SNP-Screen | T01103 from Sacace Biotechnologies is available for delivery Description: RT-PCR test for detection of MTHFR gene mutation (Ala222Val; rs1801133) for Coagulation/Fibrinolysis System Available Formats: - Ready to Use 0,2 mL...
    441-T01103
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  • FVII (G1238A) SNP-Screen

    FVII (G1238A) SNP-Screen | T01105

    Select Currency //742.00 - Select Currency //1,002.00
    FVII (G1238A) SNP-Screen | T01105 from Sacace Biotechnologies is available for delivery Description: RT-PCR test for detection of FVII gene mutation (Arg353Gln; rs6046) for Coagulation/Fibrinolysis System Available Formats: - Ready to Use 0,2 mL Tube...
    441-T01105
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  • FII Protrombin (G20210A) SNP-Screen

    FII Protrombin (G20210A) SNP-Screen (CE) | T01102

    Select Currency //742.00 - Select Currency //1,002.00
    FII Protrombin (G20210A) SNP-Screen | T01102 from Sacace Biotechnologies is available for delivery Description: RT-PCR test for detection of prothrombin F2 gene mutation (rs1799963) for Coagulation/Fibrinolysis System Available Formats: - Ready to Use...
    441-T01102
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  • FV (G1691A) Leiden SNP-Screen

    Folates Methabolism Panel

    Select Currency //608.00
    Folates Methabolism Panel | T01002-96-S from Sacace Biotechnologies is available for delivery Description: RT-PCR kit for detection of 4 mutations: MTHFR 677 C>T (Ala222Val), MTHFR 1298A>C (Glu429Ala), MTR 2756 A>G (Asp3919Gly), MTRR 66 A>G...
    441-T01002
    Select Currency //608.00
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  • West Nile Virus RT-PCR

    West Nile Virus RT-PCR (CE) | V53-50FRT

    Select Currency //741.00
    West Nile Virus RT-PCR | V53-50FRT from Sacace Biotechnologies is available for delivery Description: General information: Real Time PCR test for detection of WNV Target Disease Type: Dangerous Infections Specific Application: West Nile Virus Storage...
    441-V53-50FRT
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  • FV (G1691A) Leiden SNP-Screen

    FV (G1691A) Leiden SNP-Screen (CE) | T01101

    Select Currency //742.00 - Select Currency //1,002.00
    FV (G1691A) Leiden SNP-Screen | T01101 from Sacace Biotechnologies is available for delivery Description: RT-PCR test for detection of Leiden mutation (Arg506Gln; rs6025) for Coagulation/Fibrinolysis System Available Formats: - Ready to Use 0,2 mL Tube...
    441-T01101
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  • igScript-RT-qPCR-Kit

    igScript RT-qPCR Kit

    Select Currency //728.00 - Select Currency //1,527.20
    Description igScript™ RT-qPCR kit combines two powerful mixtures: i). igScript™ first strand cDNA synthesis kit and ii) ig SYBR® Green qPCR 2x master mix with standard buffer providing improved PCR efficiency, wider dynamic range,...
    4513/5
    Select Currency //728.00 - Select Currency //1,527.20
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  • igScript-probe-based-RT-qPCR-Kit

    igScript Probe-Based RT-qPCR Kit

    Select Currency //744.80 - Select Currency //2,873.60
    Description IgScript™ probe-based RT-qPCR kit combines two powerful mixtures: i). IgScript™ first strand cDNA synthesis kit and ii) probe-based qPCR 2x master mix with standard buffer providing improved PCR efficiency, wider dynamic range,...
    4243/5/7
    Select Currency //744.80 - Select Currency //2,873.60
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  • igScript™-Probe-Based-qPCR-master-mix

    igScript Probe-Based qPCR master mix

    Select Currency //710.00 - Select Currency //1,544.00
    Description IgScript™ probe-based qPCR 2x master mix contains IgScript™ Taq DNA polymerase, MgCl2, dNTPs, stabilizers, enhancers and low ROX reference dye with standard buffer providing improved qPCR efficiency, wider dynamic range, superior...
    4233/7/5
    Select Currency //710.00 - Select Currency //1,544.00
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  • igScript-One-Step-Probe-Based-RT-qPCR-Kit

    igScript Probe-Based One Step RT-qPCR Kit

    Select Currency //719.60 - Select Currency //5,885.60
    Description IgScript™ Probe-Based One Step RT-qPCR 2x master mix contains IgScript™ Reverse Transcriptase, Taq DNA polymerase, MgCl2, dNTPs, stabilizers and low ROX reference dye with standard buffer providing improved RT-qPCR efficiency,...
    4212/5/7/9
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  • igScript-One-Step-RT-PCR-Kit

    igScript One Step RT-PCR Kit

    Select Currency //711.20 - Select Currency //1,598.00
    Description igScript™ One Step RT-PCR kit combines two powerful mixtures: i) igScript™ Reverse Transcriptase and ii) Hot start Taq 2x master mix providing improved PCR efficiency, wider dynamic range, superior sensitivity and...
    4211/3
    Select Currency //711.20 - Select Currency //1,598.00
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  • igScript-RT-PCR-Kit

    igScript RT-PCR kit

    Select Currency //675.20 - Select Currency //1,438.40
    Description igScript™ RT-PCR Kit combines two powerful mixtures: i). 5x igScript™ master mix and ii) Hot start Taq 2x master mix with standard buffer for 2-steps RT-PCR. The two mixtures require minimal handling during reaction...
    4413/5
    Select Currency //675.20 - Select Currency //1,438.40
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  • igScript-First-Strand-cDNA-Synthesis-kit

    igScript First Strand cDNA Synthesis Kit

    Select Currency //560.00 - Select Currency //950.00
    Description igScript™ first strand cDNA synthesis kit includes 5x igScript™ master mix which contains igScript™ Reverse Transcriptase, recombinant RNase inhibitor, dNTPs, an optimized buffer, MgCl2 and protein stabilizers...
    4312/4
    Select Currency //560.00 - Select Currency //950.00
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  • PRRSV PCR-Kit

    PRRSV PCR-Kit

    Select Currency //1,383.00
    K042
    Select Currency //1,383.00
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  • TTV RT-PCR

    TTV RT-PCR

    Select Currency //280.00
    Size: 25 Tests/Kit
    HD-0013-02
    Select Currency //280.00
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  • Rubella Virus RT-PCR

    Rubella Virus RT-PCR

    Select Currency //500.00
    Size: 25 Tests/Kit
    RR-0055-02
    Select Currency //500.00
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    Select Currency //500.00
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  • Mumps Virus RT-PCR

    Mumps Virus RT-PCR

    Select Currency //500.00
    Size: 25 Tests/Kit
    RR-0056-02
    Select Currency //500.00
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    Select Currency //500.00
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  • Measles Virus RT-PCR

    Measles Virus RT-PCR

    Select Currency //500.00
    Size: 25 Tests/Kit
    RR-0054-02
    Select Currency //500.00
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  • Lassa virus RT-PCR

    Lassa virus RT-PCR

    Select Currency //651.00
    Size: 25 Tests/Kit
    QR-0222-01
    Select Currency //651.00
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